Determination of selenoprotein P in human plasma by solid phase extraction and inductively coupled plasma mass spectrometry

A method based on solid phase extraction was developed for the determination of selenoprotein P as selenium in human plasma. Separation of selenoprotein P from other selenium-containing proteins was accomplished by immobilized metal-ion affinity chromatography. The selenium content was subsequently measured by inductively coupled plasma mass spectrometry (ICP-MS) monitoring the Se-82 isotope. Linear response was observed in the concentration range 0.3-70.8 mu g/l selenium as selenoprotein P with a correlation coefficient of 0.9994. The precision expressed as relative standard deviation was better than 2% in this range. The estimated limit of detection was 2 mu g/l and the experimentally verified quantification limit was 5 mu g/l, giving a relative standard deviation less than 2%. (C) 2000 Elsevier Science B.V. All rights reserved.