Label-free optical detection of anthrax-causing spores

被引：33

作者：

Acharya, Ghanashyam

Doorneweerd, Derek D.

Chang, Chun-Li

Henne, Walter A.

Low, Philip S.

Savran, Cagri A. ^{[1
]}

机构：

[1] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA

[2] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

[3] Purdue Univ, Sch Elect & Comp Engn, W Lafayette, IN 47907 USA

[4] Purdue Univ, Sch Mech Engn, W Lafayette, IN 47907 USA

[5] Purdue Univ, Sch Biomed Engn, W Lafayette, IN 47907 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 04期

关键词：

RAPID-DETECTION; BACILLUS-ANTHRACIS; PEPTIDE LIGANDS;

D O I：

10.1021/ja0656649

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An optical biosensor integrated with short peptide ligands for detection of Bacillus anthracis spores is described. A short peptide that can bind specifically to B. anthracis spores was synthesized and used as the ligand. With this approach, a minimum of 34 spores of B. anthracis, specifically captured on the sensor, were detected by measuring the laser light transmission intensity and verified by laser scanning confocal fluorescence microscopy. The differential nature of measurement reduces the incidence of false positives. The detection process, from capturing B. anthracis spores on the sensor surface to laser transmission intensity measurements, required a short time of 35 min. This biosensor, with its simple instrumentation, ease of use, and short detection time, can enable the development of a portable device for rapid, label-free, specific, and uantitative detection of anthrax-causing spores.

引用

收藏

页码：732 / 733

页数：2

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