Annotating genomes with massive-scale RNA sequencing

被引：172

作者：

Denoeud, France ^{[1
,2
,3
]}

Aury, Jean-Marc ^{[1
,2
,3
]}

Da Silva, Corinne ^{[1
,2
,3
]}

Noel, Benjamin ^{[1
,2
,3
]}

Rogier, Odile ^{[1
,2
,3
]}

Delledonne, Massimo ^{[4
]}

Morgante, Michele ^{[5
]}

Valle, Giorgio ^{[6
]}

Wincker, Patrick ^{[1
,2
,3
]}

Scarpelli, Claude ^{[1
,2
,3
]}

Jaillon, Olivier ^{[1
,2
,3
]}

Artiguenave, Francois ^{[1
,2
,3
]}

机构：

[1] Inst Genom, DSV, CEA, F-91057 Evry, France

[2] CNRS, UMR 8030, F-91057 Evry, France

[3] Univ Evry, F-91057 Evry, France

[4] Sci & Technol Dept, I-37134 Verona, Italy

[5] Ist Genom Applicata, I-33100 Udine, Italy

[6] Univ Padua, CRIBI, I-35121 Padua, Italy

来源：

GENOME BIOLOGY | 2008年 / 9卷 / 12期

关键词：

D O I：

10.1186/gb-2008-9-12-r175

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.

引用

页数：12

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