Rapid and simple high-performance liquid chromatographic assay for the determination of metformin in human plasma and breast milk

被引：81

作者：

Zhang, M

Moore, GA

Lever, M

Gardiner, SJ

Kirkpatrick, CMJ

Begg, EJ

机构：

[1] Christchurch Hosp, Dept Clin Pharmacol, Christchurch, New Zealand

[2] Christchurch Hosp, Dept Toxicol, Christchurch, New Zealand

[3] Christchurch Hosp, Dept Biochem, Christchurch, New Zealand

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 766卷 / 01期

关键词：

metformin;

D O I：

10.1016/S0378-4347(01)00430-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and simple high-performance liquid chromatographic (HPLC) assay for the determination of metformin in human plasma and breast milk is described. After proteins were precipitated with acetonitrile, metformin and the internal standard buformin were resolved on a cation-exchange column and detected by UV detection at 236 nm. Standard curves were linear over the concentration range 20.0-4000 mug/1. Intra- and inter-day coefficients of variation were <9.0% and the limit of quantification was around 20 mug/1. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：175 / 179

页数：5

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