Injectable self-crosslinking HA-SH/Col I blend hydrogels for in vitro construction of engineered cartilage

被引：50

作者：

Chen, Yafang ^{[1
]}

Sui, Junhui ^{[1
]}

Wang, Qing ^{[1
]}

Yin, Yijia ^{[2
]}

Liu, Jun ^{[1
]}

Wang, Qiguang ^{[1
]}

Han, Xianglong ^{[2
]}

Sun, Yong ^{[1
]}

Fan, Yujiang ^{[1
]}

Zhang, Xingdong ^{[1
]}

机构：

[1] Sichuan Univ, Natl Engn Res Ctr Biomat, 29 Wangjiang Rd, Chengdu 610064, Sichuan, Peoples R China

[2] Sichuan Univ, West China Hosp Stomatol, Dept Orthodont, State Key Lab Oral Dis, 14 3rd Sect Renmin South Rd, Chengdu 610041, Sichuan, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2018年 / 190卷

基金：

中国国家自然科学基金;

关键词：

Thiolated hyaluronic acid; Collagen I; Controllable self-crosslinking; Blend hydrogel; Injectable; MESENCHYMAL STEM-CELLS; HYALURONIC-ACID HYDROGEL; CHONDROGENIC DIFFERENTIATION; TISSUE REGENERATION; STROMAL CELLS; SCAFFOLDS; COLLAGEN; CHITOSAN; DESIGN; MATRIX;

D O I：

10.1016/j.carbpol.2018.02.057

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The injectable self-crosslinking blend hydrogel by combination of collagen I and thiolated hyaluronic acid could alleviate collagen I contraction in vitro and overcome weak cell adhesive sites of hyaluronic acid. Five groups of injectable hydrogels with different ratios were prepared to investigate their gelation time, injection force, mechanical properties, swelling capacity and disintegration performance. These results indicated that Col(7)HA-SH3 hydrogel achieved the optimal controlled and injectable effect, the gelation time was just ten seconds with injection force at 3.5 N, and the storage modulus of hydrogel could reach 11 kPa with frequency at 10 Hz. Furthermore, the phenotype maintaining, biocompatibility and chondrocytes proliferation were administrated by CLSM, SEM, histological staining, immuohistochemical staining, MTT test and glycoaminoglycans quantification. Similarly, the Col7HA-SH3 blend hydrogel encapsulated chondrocytes presented most excellent proliferation potential, phenotype maintaining, biocompatibility and convenient operational characteristics. These findings might approach the underlying clinical application of blend hydrogel in cartilage repair.

引用

页码：57 / 66

页数：10

共 52 条

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The self-crosslinking smart hyaluronic acid hydrogels as injectable three-dimensional scaffolds for cells culture [J].