Expression of nitric oxide synthase in a murine model of viral myocarditis induced by coxsackievirus B3

被引：56

作者：

Mikami, S

Kawashima, S

Kanazawa, K

Hirata, K

Katayama, Y

Hotta, H

Hayashi, Y

Ito, H

Yokoyama, M

机构：

[1] KOBE UNIV, SCH MED, DEPT INTERNAL MED 1, CHUO KU, KOBE 650, JAPAN

[2] KOBE UNIV, SCH MED, DEPT MICROBIOL, CHUO KU, KOBE 650, JAPAN

[3] KOBE UNIV, SCH MED, DEPT PATHOL 1, CHUO KU, KOBE 650, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 220卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.0519

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide is known to participate in the immune and inflammatory processes. In this study, we investigated the production of nitric oxide (NO) in murine viral myocarditis induced by coxsackievirus B3. The expression of inducible NO synthase (iNOS) mRNA in the heart first appeared on day 4 after virus inoculation and it was detectable for one month by reverse transcriptase-polymerase chain reaction (RT-PCR) methods. iNOS activity which was determined by the conversion of L-[H-3] arginine to L-[H-3]citrulline was increased on day 4 and revealed its peak on day 8. Immunohistochemistry on day 7 showed increased iNOS staining mainly in infiltrating macrophages and polymorphonuclear leukocytes. Thus, NO is thought to be produced in the heart and play an important role in a murine model of coxsackievirus B3-induced viral myocarditis. (C) 1996 Academic Press, Inc.

引用

页码：983 / 989

页数：7

共 38 条

[1] ABNORMAL CONTRACTILE FUNCTION DUE TO INDUCTION OF NITRIC-OXIDE SYNTHESIS IN RAT CARDIAC MYOCYTES FOLLOWS EXPOSURE TO ACTIVATED MACROPHAGE-CONDITIONED MEDIUM [J].