The effects of adenoviral transfection of the keratinocyte growth factor gene on epidermal stem cells: An in vitro study

被引：13

作者：

Li, Xinping ^{[1
,2
]}

Liang, Ling ^{[3
]}

Zhao, Pin ^{[4
]}

Uchida, Kenzo ^{[5
]}

Baba, Hisatoshi ^{[5
]}

Huang, Hong ^{[6
]}

Bai, Wenfang ^{[1
,2
]}

Bai, Liming ^{[1
,2
]}

Zhang, Mingsheng ^{[1
,2
,3
]}

机构：

[1] Guangdong Gen Hosp, Guangdong Geriatr Inst, Dept Phys Med & Rehabil, Guangzhou 510080, Guangdong, Peoples R China

[2] Guangdong Acad Med Sci, Guangdong Geriatr Inst, Dept Phys Med & Rehabil, Guangzhou 510080, Guangdong, Peoples R China

[3] Jinan Univ, Clin Coll 1, Guangzhou 510632, Guangdong, Peoples R China

[4] Changsha Med Univ, Changsha 410219, Hunan, Peoples R China

[5] Univ Fukui, Fac Med Sci, Dept Orthopaed & Rehabil Med, Fukui 9101193, Japan

[6] Univ S Florida, Sch Informat, Bay Pines, FL 33744 USA

来源：

MOLECULES AND CELLS | 2013年 / 36卷 / 04期

关键词：

adenoviral vector; epidermal stem cells; keratinocyte growth factor; EXPRESSION; DIFFERENTIATION; THERAPY; MORPHOGENESIS; HOMEOSTASIS; EXPANSION;

D O I：

10.1007/s10059-013-0093-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Epidermal stem cells (ESCs) are characterized as slowcycling, multi-potent, and self-renewing cells that not only maintain somatic homeostasis but also participate in tissue regeneration and repair. To examine the feasibility of adenoviral vector-mediated keratinocyte growth factor (KGF) gene transfer into in vitro-expanded ESCs, ESCs were isolated from samples of human skin, cultured in vitro, and then transfected with recombinant adenovirus (Ad) carrying the human KGF gene (AdKGF) or green fluorescent protein gene (AdGFP). The effects of KGF gene transfer on cell proliferation, cell cycle arrest, cell surface antigen phenotype, and beta-catenin expression were investigated. Compared to ESCs transfected with AdGFP, AdKGFtransfected ESCs grew well, maintained a high proliferative capacity in keratinocyte serum-free medium, and expressed high levels of beta-catenin. AdKGF infection increased the number of ESCs in the G0/G1 phase and promoted ESCs entry into the G2/M phase, but had no effect on cell surface antigen phenotype (CD49f(+)/CD71(-)). The results suggest that KGF gene transfer can stimulate ESCs to grow and undergo cell division, which can be applied to enhance cutaneous wound healing.

引用

页码：316 / 321

页数：6

共 36 条

[1]

Keratinocyte growth factor induces hyperproliferation and delays differentiation in a skin equivalent model system [J].