Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter through a highly conserved region

被引:8
作者
Kelly, Lisa E.
Carrel, Tessa L.
Herman, Gail E.
El-Hodiri, Heithem M. [1 ]
机构
[1] Ohio State Univ, Columbus Childrens Res Inst, Ctr Mol & Human Genet, Columbus, OH 43205 USA
[2] Ohio State Univ, Sch Biol Sci, Program Mol Cellular & Dev Biol, Columbus, OH 43205 USA
[3] Ohio State Univ, Dept Pediat, Sch Med & Publ Hlth, Columbus, OH 43205 USA
关键词
Zic3; Pbx1; Meis1; xenopus; transgenic; promoter regulation; conserved sequence elements;
D O I
10.1016/j.bbrc.2006.03.235
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Xenopus Zic3 (zinc finger in the cerebellum-3) is expressed in the dorsal neural tube of tailbud embryos and tadpoles. We have isolated a 3.1 kb DNA fragment from the Xenopus laevis Zic3 locus that drives proper expression of a GFP reporter in transgenic frog tailbud embryos and tadpoles. This fragment contains regions that are highly similar among frogs, mice, and humans. One extremely conserved region contains a predicted Pbx binding site. We found that the transcription factors Pbx1b and Meis1 can bind this site and synergistically transactivate expression of a reporter containing the conserved region. Finally, we found that an intact Pbx site is essential for normal Zic3 promoter activity in transgenic frog embryos. Our data strongly suggest that a highly conserved region of the Zic3 promoter functions by direct interaction with Pbx1b and Meis1. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:1031 / 1037
页数:7
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