Pbx1 and Meis1 regulate activity of the Xenopus laevis Zic3 promoter through a highly conserved region

被引：8

作者：

Kelly, Lisa E.

Carrel, Tessa L.

Herman, Gail E.

El-Hodiri, Heithem M. ^{[1
]}

机构：

[1] Ohio State Univ, Columbus Childrens Res Inst, Ctr Mol & Human Genet, Columbus, OH 43205 USA

[2] Ohio State Univ, Sch Biol Sci, Program Mol Cellular & Dev Biol, Columbus, OH 43205 USA

[3] Ohio State Univ, Dept Pediat, Sch Med & Publ Hlth, Columbus, OH 43205 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 344卷 / 03期

关键词：

Zic3; Pbx1; Meis1; xenopus; transgenic; promoter regulation; conserved sequence elements;

D O I：

10.1016/j.bbrc.2006.03.235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Xenopus Zic3 (zinc finger in the cerebellum-3) is expressed in the dorsal neural tube of tailbud embryos and tadpoles. We have isolated a 3.1 kb DNA fragment from the Xenopus laevis Zic3 locus that drives proper expression of a GFP reporter in transgenic frog tailbud embryos and tadpoles. This fragment contains regions that are highly similar among frogs, mice, and humans. One extremely conserved region contains a predicted Pbx binding site. We found that the transcription factors Pbx1b and Meis1 can bind this site and synergistically transactivate expression of a reporter containing the conserved region. Finally, we found that an intact Pbx site is essential for normal Zic3 promoter activity in transgenic frog embryos. Our data strongly suggest that a highly conserved region of the Zic3 promoter functions by direct interaction with Pbx1b and Meis1. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1031 / 1037

页数：7