A promoter-trap vector for clock-controlled genes in the cyanobacterium Synechocystis sp PCC 6803

被引：28

作者：

Aoki, S

Kondo, T

Ishiura, M ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Sci, Ctr Gene Res, Nagoya, Aichi 4648602, Japan

[2] Nagoya Univ, Grad Sch Human Informat, Div Biol Informat, Nagoya, Aichi 4648601, Japan

[3] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Nagoya, Aichi 4648602, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2002年 / 49卷 / 03期

基金：

日本学术振兴会;

关键词：

circadian clock-controlled gene (ccg); luxAB; Synechocystis sp strain PCC 6803;

D O I：

10.1016/S0167-7012(01)00376-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We constructed a promoter-trap vector pPT6803-1 to isolate circadian clock-controlled promoters in the cyanobacterium Synechocystis sp. strain PCC 6803. The vector contains a promoterless luciferase gene set (luxAB) from Vibrio harveyi that is targeted to a specific site of the Synechocystis genome as a reporter for gene expression. A library was constructed in pPT6803-1 by introducing the genomic DNA fragments upstream of luxAB to transform Synechocystis cells. Of approximately 10,000 Synechocystis transformants, at least 55 (#1-55) showed circadian rhythms of bioluminescence under continuous illumination. Clones #19, #22, and #26 exhibited obviously different waveforms of bioluminescence from each other. Deletion analysis and primer extension experiments mapped the promoters for the clpP. slr1634, and rbpP genes that are responsible for biolumineseence from #19, #22, and #26, respectively. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：265 / 274

页数：10

共 24 条

[11] CIRCADIAN-RHYTHMS IN PROKARYOTES - LUCIFERASE AS A REPORTER OF CIRCADIAN GENE-EXPRESSION IN CYANOBACTERIA [J].