Identification and functional characterization of a type I signal peptidase gene of Bacillus megaterium DSM319

被引：23

作者：

Nahrstedt, H ^{[1
]}

Wittchen, KD ^{[1
]}

Rachman, MA ^{[1
]}

Meinhardt, F ^{[1
]}

机构：

[1] Univ Munster, Inst Mol Mikrobiol & Biotechnol, D-48149 Munster, Germany

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2004年 / 64卷 / 02期

关键词：

D O I：

10.1007/s00253-003-1469-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The sipM gene of Bacillus megaterium encoding a type I signal peptidase (SPase) was isolated and structurally characterized. RNA analysis revealed a transcript size in accordance with a bicistronic operon comprising sipM and an adjacent open reading frame. Inactivation of sipM by targeted gene disruption could not be achieved, indicating its essential role for cell viability since there might be no other type I SPase of major importance present in B. megaterium. Plasmid-assisted amplification of the gene resulted in an increase in activity of the heterologous glucanase used as an extracellular reporter, suggesting a potential bottleneck for protein secretion within this species.

引用

页码：243 / 249

页数：7

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