学术探索
学术期刊
新闻热点
数据分析
智能评审

The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome

被引：672
作者：
Sebaihia, Mohammed
Wren, Brendan W.
Mullany, Peter
Fairweather, Neil F.
Minton, Nigel
Stabler, Richard
Thomson, Nicholas R.
Roberts, Adam P.
Cerdeno-Tarrraga, Ana M.
Wang, Hongmei
Holden, Matthew T. G.
Wright, Anne
Churcher, Carol
Quail, Michael A.
Baker, Stephen
Bason, Nathalie
Brooks, Karen
Chillingworth, Tracey
Cronin, Ann
Davis, Paul
Dowd, Linda
Fraser, Audrey
Feltwell, Theresa
Hance, Zahra
Holroyd, Simon
Jagels, Kay
Moule, Sharon
Mungall, Karen
Price, Claire
Rabbinowitsch, Ester
Sharp, Sarah
Simmonds, Mark
Stevens, Kim
Unwin, Louise
Whithead, Sally
Dupuy, Bruno
Dougan, Gordon
Barrell, Bart
Parkhill, Julian
机构：
[1] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England
[2] London Sch Hyg & Trop Med, Dept Infect & Trop Med, London WC1E 7HT, England
[3] UCL, Eastman Dent Inst Oral Hlth Care Sci, London WC1X 8LD, England
[4] Univ London Imperial Coll Sci Technol & Med, Ctr Mol Microbiol & Infect, London SW7 2AZ, England
[5] Univ Nottingham, Inst Infect Immun & Inflammat, Ctr Biomol Sci, Nottingham NG7 2RD, England
[6] Inst Pasteur, Unite Genet Mol Bacterienne, F-75724 Paris 15, France
来源：
NATURE GENETICS | 2006年 / 38卷 / 07期
基金：
英国惠康基金;
关键词：
D O I：
10.1038/ng1830
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.
引用
收藏
页码：779 / 786
页数：8
相关论文
共 50 条
[1]   THE VANZ GENE OF TN1546 FROM ENTEROCOCCUS-FAECIUM BM4147 CONFERS RESISTANCE TO TEICOPLANIN [J].
ARTHUR, M ;
DEPARDIEU, F ;
MOLINAS, C ;
REYNOLDS, P ;
COURVALIN, P .
GENE, 1995, 154 (01) :87-92
[2]   The interaction between bacteria and bile [J].
Begley, M ;
Gahan, CGM ;
Hill, C .
FEMS MICROBIOLOGY REVIEWS, 2005, 29 (04) :625-651
[3]   Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp atroseptica and characterization of virulence factors [J].
Bell, KS ;
Sebaihia, M ;
Pritchard, L ;
Holden, MTG ;
Hyman, LJ ;
Holeva, MC ;
Thomson, NR ;
Bentley, SD ;
Churcher, LJC ;
Mungall, K ;
Atkin, R ;
Bason, N ;
Brooks, K ;
Chillingworth, T ;
Clark, K ;
Doggett, J ;
Fraser, A ;
Hance, Z ;
Hauser, H ;
Jagels, K ;
Moule, S ;
Norbertczak, H ;
Ormond, D ;
Price, C ;
Quail, MA ;
Sanders, M ;
Walker, D ;
Whitehead, S ;
Salmond, GPC ;
Birch, PRJ ;
Parkhill, J ;
Toth, IK .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (30) :11105-11110
[4]   MUCOSAL ASSOCIATION BY CLOSTRIDIUM-DIFFICILE IN THE HAMSTER GASTROINTESTINAL-TRACT [J].
BORRIELLO, SP ;
WELCH, AR ;
BARCLAY, FE ;
DAVIES, HA .
JOURNAL OF MEDICAL MICROBIOLOGY, 1988, 25 (03) :191-196
[5]   A chimeric ribozyme in Clostridium difficile combines features of group I introns and insertion elements [J].
Braun, V ;
Mehlig, M ;
Moos, M ;
Rupnik, M ;
Kalt, B ;
Mahony, DE ;
von Eichel-Streiber, C .
MOLECULAR MICROBIOLOGY, 2000, 36 (06) :1447-1459
[6]   Molecular and physiological characterisation of spore germination in Clostridium botulinum and C-sporogenes [J].
Broussolle, V ;
Alberto, F ;
Shearman, CA ;
Mason, DR ;
Botella, L ;
Nguyen-The, C ;
Peck, MW ;
Carlin, F .
ANAEROBE, 2002, 8 (03) :89-100
[7]   The genome sequence of Clostridium tetani, the causative agent of tetanus disease [J].
Brüggemann, H ;
Bäumer, S ;
Fricke, WF ;
Wiezer, A ;
Liesegang, H ;
Decker, I ;
Herzberg, C ;
Martínez-Arias, R ;
Merkl, R ;
Henne, A ;
Gottschalk, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (03) :1316-1321
[8]   Conjugative transposons:: the tip of the iceberg [J].
Burrus, V ;
Pavlovic, G ;
Decaris, B ;
Guédon, G .
MOLECULAR MICROBIOLOGY, 2002, 46 (03) :601-610
[9]   Molecular characterization of the surface layer proteins from Clostridium difficile [J].
Calabi, E ;
Ward, S ;
Wren, B ;
Paxton, T ;
Panico, M ;
Morris, H ;
Dell, A ;
Dougan, G ;
Fairweather, N .
MOLECULAR MICROBIOLOGY, 2001, 40 (05) :1187-1199
[10]   Quorum sensing in Clostridium difficile:: analysis of a luxS-type signalling system [J].
Carter, GP ;
Purdy, D ;
Williams, P ;
Minton, NP .
JOURNAL OF MEDICAL MICROBIOLOGY, 2005, 54 (02) :119-127
12345