Peracetylated β-cyclodextrin as additive in enzymatic reactions:: enhanced reaction rate and enantiomeric ratio in lipase-catalyzed transesterifications in organic solvents

被引：34

作者：

Ghanem, A ^{[1
]}

Schurig, V ^{[1
]}

机构：

[1] Univ Tubingen, Inst Organ Chem, D-72076 Tubingen, Germany

来源：

TETRAHEDRON-ASYMMETRY | 2001年 / 12卷 / 19期

关键词：

D O I：

10.1016/S0957-4166(01)00482-7

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Peracetylated beta -cyclodextrin has been employed as a macrocyclic additive to enhance the enantiomeric ratio E and reaction rate in Pseudomonas cepacia lipase (PSL)-catalyzed enantioselective transesterification of 1-(2-furyl)ethanol in organic solvents, The beneficial action or the cyclodextrin used as a regulator of lipase was tentatively interpreted as increasing the conformational flexibility of the enzyme and undergoing host-guest complexation with the product. thereby preventing product inhibition and leading to an enhancement of the enantiomeric ratio E and the reaction rate. The effect of the organic solvent on the present cyclodextrin-mediated enzymatic transesterification has been studied. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2761 / 2766

页数：6

共 34 条

[31] Enhancement of selectivity and reactivity of lipases by additives [J].

Theil, F .

TETRAHEDRON, 2000, 56 (19) :2905-2919

[32] SOLVENT VARIATION INVERTS SUBSTRATE-SPECIFICITY OF AN ENZYME [J].

WESCOTT, CR ;

KLIBANOV, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (05) :1629-1631

[33] PH-DEPENDENCE OF SUBTILISIN DISPERSED IN ORGANIC-SOLVENTS [J].

YANG, Z ;

ZACHERL, D ;

RUSSELL, AJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (26) :12251-12257

[34]

YE H, 1992, J AM CHEM SOC, V144, P5470

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