Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection

被引：73

作者：

Kamachi, Kazunari

Toyoizumi-Ajisaka, Hiromi

Toda, Kohei

Soeung, Sann Chan

Sarath, Svay

Nareth, Ya

Horiuchi, Yoshinobu

Kojima, Kazunobu

Takahashi, Motohide

Arakawa, Yoshichika

机构：

[1] Natl Inst Infect Dis, Dept Bacterial Pathogenesis & Infect Control, Musashimurayma, Tokyo 2080011, Japan

[2] Minist Hlth, World Hlth Org Representat Off, Phnom Penh, Cambodia

[3] Minist Hlth, Dept Natl Immunizat Program, Phnom Penh, Cambodia

[4] World Hlth Org Western Pacific Reg Off, Manila, Philippines

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 05期

关键词：

D O I：

10.1128/JCM.44.5.1899-1902.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.

引用

页码：1899 / 1902

页数：4

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