Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection

被引：73

作者：

Kamachi, Kazunari

Toyoizumi-Ajisaka, Hiromi

Toda, Kohei

Soeung, Sann Chan

Sarath, Svay

Nareth, Ya

Horiuchi, Yoshinobu

Kojima, Kazunobu

Takahashi, Motohide

Arakawa, Yoshichika

机构：

[1] Natl Inst Infect Dis, Dept Bacterial Pathogenesis & Infect Control, Musashimurayma, Tokyo 2080011, Japan

[2] Minist Hlth, World Hlth Org Representat Off, Phnom Penh, Cambodia

[3] Minist Hlth, Dept Natl Immunizat Program, Phnom Penh, Cambodia

[4] World Hlth Org Western Pacific Reg Off, Manila, Philippines

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 05期

关键词：

D O I：

10.1128/JCM.44.5.1899-1902.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.

引用

页码：1899 / 1902

页数：4

共 26 条

[21] Loop-mediated isothermal amplification of DNA [J].

Notomi, Tsugunori ;

Okayama, Hiroto ;

Masubuchi, Harumi ;

Yonekawa, Toshihiro ;

Watanabe, Keiko ;

Amino, Nobuyuki ;

Hase, Tetsu .

NUCLEIC ACIDS RESEARCH, 2000, 28 (12)

[22]

Nygren M, 2000, J CLIN MICROBIOL, V38, P55

[23] Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica [J].

Parkhill, J ;

Sebaihia, M ;

Preston, A ;

Murphy, LD ;

Thomson, N ;

Harris, DE ;

Holden, MTG ;

Churcher, CM ;

Bentley, SD ;

Mungall, KL ;

Cerdeño-Tárraga, AM ;

Temple, L ;

James, K ;

Harris, B ;

Quail, MA ;

Achtman, M ;

Atkin, R ;

Baker, S ;

Basham, D ;

Bason, N ;

Cherevach, I ;

Chillingworth, T ;

Collins, M ;

Cronin, A ;

Davis, P ;

Doggett, J ;

Feltwell, T ;

Goble, A ;

Hamlin, N ;

Hauser, H ;

Holroyd, S ;

Jagels, K ;

Leather, S ;

Moule, S ;

Norberczak, H ;

O'Neil, S ;

Ormond, D ;

Price, C ;

Rabbinowitsch, E ;

Rutter, S ;

Sanders, M ;

Saunders, D ;

Seeger, K ;

Sharp, S ;

Simmonds, M ;

Skelton, J ;

Squares, R ;

Squares, S ;

Stevens, K ;

Unwin, L .

NATURE GENETICS, 2003, 35 (01) :32-40

[24] Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii [J].

Reischl, U ;

Lehn, N ;

Sanden, GN ;

Loeffelholz, MJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (05) :1963-1966

[25] Evaluation of real-time PCR for detection of and discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for clinical diagnosis [J].

Templeton, KE ;

Scheltinga, SA ;

van der Zee, A ;

Diederen, BMW ;

Kruijssen, AM ;

Goossens, H ;

Kuijper, E ;

Claas, ECJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (09) :4121-4126

[26] Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus [J].

Thai, HTC ;

Le, MQ ;

Vuong, CD ;

Parida, M ;

Minekawa, H ;

Notomi, T ;

Hasebe, F ;

Morita, K .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) :1956-1961

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