Germline Competent Embryonic Stem Cells Derived from Rat Blastocysts

被引：553

作者：

Li, Ping ^{[1
,7
]}

Tong, Chang ^{[1
]}

Mehrian-Shai, Ruty ^{[2
,3
]}

Jia, Li ^{[6
]}

Wu, Nancy ^{[4
]}

Yan, Youzhen ^{[4
]}

Maxson, Robert E. ^{[2
,4
]}

Schulze, Eric N. ^{[1
]}

Song, Houyan ^{[7
]}

Hsieh, Chih-Lin ^{[2
,6
]}

Pera, Martin F. ^{[1
,5
]}

Ying, Qi-Long ^{[1
,5
]}

机构：

[1] Univ So Calif, Keck Sch Med, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, Los Angeles, CA 90033 USA

[2] Univ So Calif, Keck Sch Med, Dept Biochem & Mol Biol, Los Angeles, CA 90033 USA

[3] Univ So Calif, Keck Sch Med, Custom Microarray Core Facil, Inst Med Genet, Los Angeles, CA 90033 USA

[4] Univ So Calif, Keck Sch Med, Knockout Core Facil, Norris Canc Ctr Transgen, Los Angeles, CA 90033 USA

[5] Univ So Calif, Keck Sch Med, Dept Cell & Neurobiol, Los Angeles, CA 90033 USA

[6] Univ So Calif, Keck Sch Med, Norris Canc Ctr, Dept Urol, Los Angeles, CA 90033 USA

[7] Fudan Univ, Shanghai Med Coll, Minist Educ, Key Lab Mol Med, Shanghai 20032, Peoples R China

来源：

CELL | 2008年 / 135卷 / 07期

关键词：

D O I：

10.1016/j.cell.2008.12.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rats have important advantages over mice as an experimental system for physiological and pharmacological investigations. The lack of rat embryonic stem (ES) cells has restricted the availability of transgenic technologies to create genetic models in this species. Here, we show that rat ES cells can be efficiently derived, propagated, and genetically manipulated in the presence of small molecules that specifically inhibit GSK3, MEK, and FGF receptor tyrosine kinases. These rat ES cells express pluripotency markers and retain the capacity to differentiate into derivatives of all three germ layers. Most importantly, they can produce high rates of chimerism when reintroduced into early stage embryos and can transmit through the germline. Establishment of authentic rat ES cells will make possible sophisticated genetic manipulation to create models for the study of human diseases.

引用

页码：1299 / 1310

页数：12

共 49 条

[1] A bivalent chromatin structure marks key developmental genes in embryonic stem cells [J].

Bernstein, BE ;

Mikkelsen, TS ;

Xie, XH ;

Kamal, M ;

Huebert, DJ ;

Cuff, J ;

Fry, B ;

Meissner, A ;

Wernig, M ;

Plath, K ;

Jaenisch, R ;

Wagschal, A ;

Feil, R ;

Schreiber, SL ;

Lander, ES .

CELL, 2006, 125 (02) :315-326

[2] Rat embryonic stem cells: A progress report [J].

Brenin, D ;

Look, J ;

Bader, M ;

Hubner, N ;

Levan, G ;

Iannaccone, P .

TRANSPLANTATION PROCEEDINGS, 1997, 29 (03) :1761-1765

[3] Derivation of pluripotent epiblast stem cells from mammalian embryos [J].

Brons, I. Gabrielle M. ;

Smithers, Lucy E. ;

Trotter, Matthew W. B. ;

Rugg-Gunn, Peter ;

Sun, Bowen ;

de Sousa Lopes, Susana M. Chuva ;

Howlett, Sarah K. ;

Clarkson, Amanda ;

Ahrlund-Richter, Lars ;

Pedersen, Roger A. ;

Vallier, Ludovic .

NATURE, 2007, 448 (7150) :191-U7

[4] The origin and efficient derivation of embryonic stem cells in the mouse [J].

Brook, FA ;

Gardner, RL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (11) :5709-5712

[5] Rapid loss of Oct-4 and pluripotency in cultured rodent blastocysts and derivative cell lines [J].

Buehr, M ;

Nichols, J ;

Stenhouse, F ;

Mountford, P ;

Greenhalgh, CJ ;

Kantachuvesiri, S ;

Brooker, G ;

Mullins, J ;

Smith, AG .

BIOLOGY OF REPRODUCTION, 2003, 68 (01) :222-229

[6] Gene targeting in mice: functional analysis of the mammalian genome for the twenty-first century [J].

Capecchi, MR .

NATURE REVIEWS GENETICS, 2005, 6 (06) :507-512

[7] Enhanced plating efficiency of trypsin-adapted human embryonic stem cells is reversible and independent of trisomy 12/17 [J].

Chan, Elayne M. ;

Yates, Frank ;

Boyer, Leah F. ;

Schlaeger, Thorsten M. ;

Daley, George Q. .

CLONING AND STEM CELLS, 2008, 10 (01) :107-117

[8] LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells [J].

Dahéron, L ;

Opitz, SL ;

Zaehres, H ;

Lensch, WM ;

Andrews, PW ;

Itskovitz-Eldor, J ;

Daley, GQ .

STEM CELLS, 2004, 22 (05) :770-778

[9] YOLK-SAC CARCINOMA GROWN FROM RAT EGG CYLINDERS [J].

DAMJANOV, I ;

SELL, S .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1977, 58 (05) :1523-1525

[10] Specificity and mechanism of action of some commonly used protein kinase inhibitors [J].

Davies, SP ;

Reddy, H ;

Caivano, M ;

Cohen, P .

BIOCHEMICAL JOURNAL, 2000, 351 (351) :95-105

← 1 2 3 4 5 →