Plant-produced hepatitis B core protein chimera carrying anthrax protective antigen domain-4

被引：10

作者：

Bandurska, Katarzyna ^{[1
]}

Brodzik, Robert ^{[1
]}

Spitsin, Sergei ^{[1
]}

Kohl, Thomas ^{[1
]}

Portocarrero, Carla ^{[1
]}

Smirnov, Yuri ^{[1
]}

Pogrebnyak, Natalia ^{[1
]}

Sirko, Agnieszka ^{[2
]}

Koprowski, Hilary ^{[1
]}

Golovkin, Maxim ^{[1
]}

机构：

[1] Thomas Jefferson Univ, Biotechnol Fdn Labs, Philadelphia, PA 19107 USA

[2] Polish Acad Sci, Inst Biochem & Biophys, Warsaw, Poland

来源：

HYBRIDOMA | 2008年 / 27卷 / 04期

关键词：

D O I：

10.1089/hyb.2008.0008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The hepatitis B core antigen (HBcAg) can generate a strong immune response and is recognized as an effective carrier for foreign epitopes. The domain-4 epitope of the anthrax protective antigen (PA-D4) plays an essential role in generating protective immunity against virulent Bacillus anthracis. Here we report the successful production of a recombinant protein comprised of the antigenic PA-D4 integrated into the c/e1 loop of HBcAg in transgenic low-alkaloid Nicotiana tabacum. Sera of mice injected with the plant-derived purified HB/PA-D4 protein exhibited significant anti-PA- and anti-HBcAg-specific IgG titers; however, formation of virus-like particles (VLP) was not observed. These data support the feasibility of producing complex protein chimeras in plants.

引用

收藏

页码：241 / 247

页数：7

相关论文

共 37 条

[11] PHYSICOCHEMICAL ANALYSIS OF THE HEPATITIS-B VIRUS CORE ANTIGEN PRODUCED BY A BACULOVIRUS EXPRESSION VECTOR [J].

HILDITCH, CM ;

ROGERS, LJ ;

BISHOP, DHL .

JOURNAL OF GENERAL VIROLOGY, 1990, 71 :2755-2759

[12] ANALYSIS OF AGROBACTERIUM-TUMEFACIENS VIRULENCE MUTANTS IN LEAF-DISKS [J].

HORSCH, RB ;

KLEE, HJ ;

STACHEL, S ;

WINANS, SC ;

NESTER, EW ;

ROGERS, SG ;

FRALEY, RT .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (08) :2571-2575

[13] Immunogenicity of the epitope of the foot-and-mouth disease virus fused with a hepatitis B core protein as expressed in transgenic tobacco [J].

Huang, YH ;

Liang, WQ ;

Wang, YJ ;

Zhou, ZA ;

Pan, AH ;

Yang, XH ;

Huang, C ;

Chen, JX ;

Zhang, DB .

VIRAL IMMUNOLOGY, 2005, 18 (04) :668-677

[14] Rapid, high-level production of hepatitis B core antigen in plant leaf and its immunogenicity in mice [J].

Huang, Z ;

Santi, L ;

LePore, K ;

Kilbourne, J ;

Arntzen, CJ ;

Mason, HS .

VACCINE, 2006, 24 (14) :2506-2513

[15] EXPRESSION MECHANISM OF THE HEPATITIS-B VIRUS (HBV) C-GENE AND BIOSYNTHESIS OF HBE ANTIGEN [J].

JEANJEAN, O ;

LEVRERO, M ;

WILL, H ;

PERRICAUDET, M ;

ROSSIGNOL, JM .

VIROLOGY, 1989, 170 (01) :99-106

[16] Plant-produced cottontail rabbit papillomavirus L1 protein protects against tumor challenge: a proof-of-concept study [J].

Kohl, T. ;

Hitzeroth, I. I. ;

Stewart, D. ;

Varsani, A. ;

Govan, V. A. ;

Christensen, N. D. ;

Williamson, A. -L. ;

Rybicki, E. P. .

CLINICAL AND VACCINE IMMUNOLOGY, 2006, 13 (08) :845-853

[17] Expression of HPV-11 L1 protein in transgenic Arabidopsis thaliana and Nicotiana tabacum [J].

Kohl, Thomas O. ;

Hitzeroth, Inga I. ;

Christensen, Neil D. ;

Rybicki, Edward P. .

BMC BIOTECHNOLOGY, 2007, 7 (1)

[18] Vaccines and sera through plant biotechnology [J].

Koprowski, H .

VACCINE, 2005, 23 (15) :1757-1763

[19] Native display of complete foreign protein domains on the surface of hepatitis B virus capsids [J].

Kratz, PA ;

Böttcher, B ;

Nassal, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (05) :1915-1920

[20] Mucosal immunogenicity of the hepatitis B core antigen [J].

Lobaina, Y ;

García, D ;

Abreu, N ;

Muzio, V ;

Aguilar, JC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 300 (03) :745-750

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