Two classes of endogenous small RNAs in Tetrahymena thermophila

被引：82

作者：

Lee, SR ^{[1
]}

Collins, K ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

GENES & DEVELOPMENT | 2006年 / 20卷 / 01期

关键词：

Tetrahymena; small RNA; RNAi; Dicer; genome rearrangement;

D O I：

10.1101/gad.1377006

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Endogenous small RNAs function in RNA interference (RNAi) pathways to guide RNA cleavage, translational repression, or methylation of DNA or chromatin. In Tetrahymena thermophila, developmentally regulated DNA elimination is governed by an RNAi mechanism involving similar to 27-30-nucleotide (nt) RNAs. Here we characterize the sequence features of the similar to 27-30-nt RNAs and a similar to 23-24-nt RNA class representing a second RNAi pathway. The similar to 23-24-nt RNAs accumulate strain-specifically manner and map to the genome in clusters that are antisense to predicted genes. These findings reveal the existence of distinct endogenous RNAi pathways in the unicellular T. thermophila, a complexity previously demonstrated only in multicellular organisms.

引用

页码：28 / 33

页数：6

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