Tanshinone IIA and astragaloside IV promote the migration of mesenchymal stem cells by up-regulation of CXCR4

被引：72

作者：

Xie, Juan ^{[1
]}

Wang, Huan ^{[2
]}

Song, Tiebing ^{[3
]}

Wang, Zongren ^{[1
]}

Li, Feng ^{[1
]}

Ma, Jing ^{[1
]}

Chen, Jun ^{[1
]}

Nan, Yayun ^{[1
]}

Yi, Hui ^{[1
]}

Wang, Wen ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Dept Tradit Chinese Med, Xijing Hosp, Xian 710032, Shanxi Province, Peoples R China

[2] Fourth Mil Med Univ, Tangdu Hosp, Dept Dermatol, Xian 710038, Shanxi Province, Peoples R China

[3] Xian Tradit Chinese Med Hosp, Dept Orthoped, Xian 710001, Shanxi Province, Peoples R China

来源：

PROTOPLASMA | 2013年 / 250卷 / 02期

关键词：

Tanshinone IIA; Astragaloside IV; Mesenchymal stem cells; Migration; CXCR4; BONE-MARROW; STROMAL CELLS; CHEMOKINE RECEPTORS; CARDIAC MYOCYTES; IN-VIVO; MYOCARDIUM; FACTOR-1-ALPHA; REGENERATION; ENGRAFTMENT; EXPRESS;

D O I：

10.1007/s00709-012-0435-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Mesenchymal stem cells (MSCs) have a therapeutic potential to treat cardiovascular diseases. However, a significant barrier to MSC therapy is insufficient MSC engraftment in ischemic myocardium after systemic administration. Here, we investigated the modulatory effects of tanshinone IIA and astragaloside IV on the migration of MSCs and further defined the underlying mechanisms. CXCR4 expression in MSCs was determined by using flow cytometry, real-time PCR, and western blotting. The results showed that CXCR4 expression was significantly higher in tanshinone IIA- and astragaloside IV-stimulated MSCs than that of the control. MSC migration toward stromal cell-derived factor-1 alpha (SDF-1 alpha) was studied using a transwell system. MSCs treated with tanshinone IIA and astragaloside IV showed stronger migration than that of the control. Moreover, this enhanced migration ability was abrogated by a CXCR4 inhibitor. In a rat acute myocardial infarction model, MSCs stimulated with tanshinone IIA and astragaloside IV were stained with Dio and injected into model rats via the tail vein. Dio-labeled cells in myocardium sections were observed by fluorescence microscopy. Tanshinone IIA- and astragaloside IV-stimulated MSCs showed enhanced capacities to home to ischemic myocardium sites. In addition, there was no significant difference in the SDF-1 alpha expression among groups. These data suggest that tanshinone IIA and astragaloside IV regulate MSC mobilization, at least partially via modulation of the CXCR4 expression.

引用

页码：521 / 530

页数：10

共 31 条

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