Qualitative and Quantitative Expression Status of the Human Chromosome 20 Genes in Cancer Tissues and the Representative Cell Lines

被引：17

作者：

Wang, Quanhui ^{[1
,2
]}

Wen, Bo ^{[2
]}

Yan, Guangrong ^{[3
]}

Wei, Junying ^{[4
,5
]}

Xie, Liqi ^{[6
,7
]}

Xu, Shaohang ^{[2
]}

Jiang, Dahai ^{[2
]}

Wang, Tingyou ^{[2
]}

Lin, Liang ^{[2
]}

Zi, Jin ^{[2
]}

Zhang, Ju ^{[1
]}

Zhou, Ruo ^{[2
]}

Zhao, Haiyi ^{[2
]}

Ren, Zhe ^{[2
]}

Qu, Nengrong ^{[2
]}

Lou, Xiaomin ^{[1
]}

Sun, Haidan ^{[1
]}

Du, Chaoqin ^{[2
]}

Chen, Chuangbin ^{[2
]}

Zhang, Shenyan ^{[1
]}

Tan, Fengji ^{[2
]}

Xian, Youqi ^{[2
]}

Gao, Zhibo ^{[2
]}

He, Minghui ^{[2
]}

Chen, Longyun ^{[2
]}

Zhao, Xiaohang ^{[8
,9
]}

Xu, Ping ^{[4
,5
]}

Zhu, Yunping ^{[4
,5
]}

Yin, Xingfeng ^{[3
]}

Shen, Huali ^{[6
,7
]}

Zhang, Yang ^{[6
,7
]}

Jiang, Jing ^{[4
,5
]}

Zhang, Chengpu ^{[4
,5
]}

Li, Liwei ^{[4
,5
]}

Chang, Cheng ^{[4
,5
]}

Ma, Jie ^{[4
,5
]}

Yan, Guoquan ^{[6
,7
]}

Yao, Jun ^{[6
,7
]}

Lu, Haojie ^{[6
,7
]}

Ying, Wantao ^{[4
,5
]}

Zhong, Fan ^{[6
,7
]}

He, Qing-Yu ^{[3
]}

Liu, Siqi ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Beijing Inst Genom, Beijing 101318, Peoples R China

[2] BGI Shenzhen, Shenzhen 518083, Peoples R China

[3] Jinan Univ, Inst Life & Hlth Engn, Guangdong Higher Educ Inst, Key Lab Funct Prot Res, Guangzhou 510632, Guangdong, Peoples R China

[4] Beijing Inst Radiat Med, Beijing Proteome Res Ctr, State Key Lab Prote, Beijing 102206, Peoples R China

[5] Natl Engn Res Ctr Prot Drugs, Beijing 102206, Peoples R China

[6] Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China

[7] Fudan Univ, Dept Chem, Shanghai 200032, Peoples R China

[8] Chinese Acad Med Sci, Canc Inst & Hosp, State Key Lab Mol Oncol, Beijing 100021, Peoples R China

[9] Peking Union Med Coll, Beijing 100021, Peoples R China

来源：

JOURNAL OF PROTEOME RESEARCH | 2013年 / 12卷 / 01期

关键词：

proteome; chromosome; C-HPP; chromosome features; quantitative correlations; GENOME-WIDE ASSOCIATION; COPY NUMBER ABERRATIONS; COLORECTAL-CANCER; MYELOID MALIGNANCIES; SUSCEPTIBILITY LOCI; PROTEOME; IDENTIFICATIONS; QUANTIFICATION; PROLIFERATION; AMPLIFICATION;

D O I：

10.1021/pr3008336

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Under the guidance of the Chromosome-centric Human Proteome Project (C-HPP),(1,2) we conducted a systematic survey of the expression status of genes located at human chromosome 20 (Chr.20) in three cancer tissues, gastric, colon, and liver carcinoma, and their representative cell lines. We have globally profiled proteomes in these samples with combined technology of LC-MS/MS and acquired the corresponding mRNA information upon RNA-seq and RNAchip. In total, 323 unique proteins were identified, covering 60% of the coding genes (323/547) in Chr.20. With regards to qualitative information of proteomics, we overall evaluated the correlation of the identified Chr.20 proteins with target genes of transcription factors or of microRNA, conserved genes and cancer-related genes. As for quantitative information, the expression abundances of Chr.20 genes were found to be almost consistent in both tissues and cell lines of mRNA in all individual chromosome regions, whereas those of Chr.20 proteins in cells are different from tissues, especially in the region of 20q13.33. Furthermore, the abundances of Chr.20 proteins were hierarchically evaluated according to tissue- or cancer-related distribution. The analysis revealed several cancer-related proteins in Chr.20 are tissue- or cell-type dependent. With integration of all the acquired data, for the first time we established a solid database of the Chr.20 proteome.

引用

页码：151 / 161

页数：11

共 48 条

[1] Affinity proteomics for systematic protein profiling of chromosome 21 gene products in human tissues [J].

Agaton, C ;

Galli, J ;

Guthenberg, IH ;

Janzon, L ;

Hansson, M ;

Asplund, A ;

Brundell, E ;

Lindberg, S ;

Ruthberg, I ;

Wester, K ;

Wurtz, D ;

Höög, C ;

Lundeberg, J ;

Ståhl, S ;

Pontén, F ;

Uhlén, M .

MOLECULAR & CELLULAR PROTEOMICS, 2003, 2 (06) :405-414

[2] A Histone Map of Human Chromosome 20q13.12 [J].

Akan, Pelin ;

Sahlen, Martin ;

Deloukas, Panagiotis .

PLOS ONE, 2009, 4 (02)

[3] Molecular cytogenetic analysis of prostatic adenocarcinomas from screening studies -: Early cancers may contain aggressive genetic features [J].

Alers, JC ;

Krijtenburg, PJ ;

Vis, AN ;

Hoedemaeker, RF ;

Wildhagen, MF ;

Hop, WCJ ;

van der Kwast, TH ;

Schröder, FH ;

Tanke, HJ ;

van Dekken, H .

AMERICAN JOURNAL OF PATHOLOGY, 2001, 158 (02) :399-406

[4]

[Anonymous], MOL CELL PROTEOMICS

[5]

Antonacopoulou AG, 2008, ANTICANCER RES, V28, P1221

[6] Prion Protein Expression and the M129V Polymorphism of the PRNP Gene in Patients With Colorectal Cancer [J].

Antonacopoulou, Anna G. ;

Palli, Maria ;

Marousi, Stella ;

Dimitrakopoulos, Fotinos-Ioannis ;

Kyriakopoulou, Urania ;

Tsamandas, Athanasios C. ;

Scopa, Chrisoula D. ;

Papavassiliou, Athanasios G. ;

Kalofonos, Haralabos P. .

MOLECULAR CARCINOGENESIS, 2010, 49 (07) :693-699

[7] CSE1L/CAS: Its role in proliferation and apoptosis [J].

Behrens, P ;

Brinkmann, U ;

Wellmann, A .

APOPTOSIS, 2003, 8 (01) :39-44

[8] Chromosome 20 deletions in myeloid malignancies: reduction of the common deleted region, generation of a PAC/BAC contig and identification of candidate genes [J].

Bench, AJ ;

Nacheva, EP ;

Hood, TL ;

Holden, JL ;

French, L ;

Swanton, S ;

Champion, KM ;

Li, J ;

Whittaker, P ;

Stavrides, G ;

Hunt, AR ;

Huntly, BJP ;

Campbell, LJ ;

Bentley, DR ;

Deloukas, P ;

Green, AR .

ONCOGENE, 2000, 19 (34) :3902-3913

[9] High resolution analysis of DNA copy-number aberrations of chromosomes 8, 13, and 20 in gastric cancers [J].

Buffart, Tineke E. ;

van Grieken, Nicole C. T. ;

Tijssen, Marianne ;

Coffa, Jordy ;

Ylstra, Bauke ;

Grabsch, Heike I. ;

van de Velde, Cornelis J. H. ;

Carvalho, Beatriz ;

Meijer, Gerrit A. .

VIRCHOWS ARCHIV, 2009, 455 (03) :213-223

[10] Deletion at chromosome band 20p 12.1 in colorectal cancer revealed by high resolution array comparative genomic hybridization [J].

Davison, EJ ;

Tarpey, PS ;

Fiegler, H ;

Tomlinson, IPM ;

Carter, NP .

GENES CHROMOSOMES & CANCER, 2005, 44 (04) :384-391

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