Effects of soluble CD4 on simian immunodeficiency virus infection of CD4-positive and CD4-negative

被引:51
作者
Schenten, D
Marcon, L
Karlsson, GB
Parolin, C
Kodama, T
Gerard, N
Sodroski, J
机构
[1] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Canc Immunol & AIDS, Boston, MA 02115 USA
[2] Univ Padua, Sch Med, Inst Microbiol, I-35121 Padua, Italy
[3] Oregon Reg Primate Res Ctr, Beaverton, OR 97006 USA
[4] Childrens Hosp, Ina Sue Perlmutter Lab, Boston, MA USA
[5] Beth Israel Deaconess Med Ctr, Dept Med, Boston, MA USA
[6] Beth Israel Deaconess Med Ctr, Dept Pediat, Boston, MA USA
[7] Harvard Univ, Sch Publ Hlth, Dept Immunol & Infect Dis, Boston, MA 02115 USA
关键词
D O I
10.1128/JVI.73.7.5373-5380.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A soluble form of the CD4 receptor (sCD4) can either enhance or inhibit the infection of cells by simian immunodeficiency virus (SIV) and human immunodeficiency virus. We investigated the basis for these varying effects by studying the entry of three SN isolates into CD4-positive and CD4-negative cells expressing different chemokine receptors. Infection of CD4-negative cells depended upon the viral envelope glycoproteins and upon the chemokine receptor, with CCR5 and gpr15 being more efficient than STRL33. Likewise, enhancement of infection by sCD4 was observed when CCR5- and gpr15-expressing target cells were used but not when those expressing STRL33 were used. The sCD4-mediated enhancement of virus infection of CD4-negative, CCR5-positive cells was related to the sCD4-induced increase in binding of the viral gp120 envelope glycoprotein to CCR5. Inhibitory effects of sCD4 could largely be explained by competition for virus attachment to cellular CD4 rather than other detrimental effects on virus infectivity (e.g., disruption of the envelope glycoprotein spike). Consistent with this, the sCD4-activated SIV envelope glycoprotein intermediate on the virus was long-lived. Thus, the net effect of sCD4 on SIV infectivity appears to depend upon the degree of enhancement of chemokine receptor binding and upon the efficiency of competition for cellular CD4.
引用
收藏
页码:5373 / 5380
页数:8
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