Mutations affecting the Rossman fold of isoleucyl-tRNA synthetase are correlated with low-level mupirocin resistance in Staphylococcus aureus

被引:85
作者
Antonio, M
McFerran, N
Pallen, MJ [1 ]
机构
[1] Univ Birmingham, Sch Med, Div Immun & Infect, Microbial Genom & Pathogenesis Unit, Birmingham B15 2TT, W Midlands, England
[2] Queens Univ Belfast, Dept Biochem, Belfast BT12 6BN, Antrim, North Ireland
关键词
D O I
10.1128/AAC.46.2.438-442.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The isoleucyl-tRNA synthetase (ileS) gene was sequenced in toto from 9 and in part from 31 Staphylococcus aureus strains with carious degrees of susceptibility to mupirocin. All strains for which the mupirocin MIC was greater than 8 mug/ml contained point mutations affecting the Rossman fold via Val-to-Phe changes at either residue 588 (V588F) or residue 631 (V631F). The importance of the V588F mutation was confirmed by an allele-specific PCR survey of 32 additional strains. Additional mutations of uncertain significance were found in residues clustered on the surface of the IleS protein.
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页码:438 / 442
页数:5
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