Real-time quantitative assay of telomerase activity using the duplex scorpion primer

被引：10

作者：

Huang, YP

Kong, DM

Yang, Y

Niu, RF

Shen, HX ^{[1
]}

Mi, HF

机构：

[1] Nankai Univ, Sch Chem, State Key Lab Funct Polymer Mat Adsorpt & Separat, Tianjin 300071, Peoples R China

[2] Nankai Univ, Sch Med, Tianjin 300071, Peoples R China

[3] Tianjin Med Univ, Canc Hosp, Oncol Ctr Lab, Tianjin 300070, Peoples R China

[4] Tianjin Med Univ, Coll Pharm, Tianjin 300070, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 11期

基金：

中国国家自然科学基金;

关键词：

duplex scorpion primer; real-time quantitative PCR; telomerase activity; TRAP assay;

D O I：

10.1023/B:bile.0000025898.15309.0e

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel real-time quantitative method for measuring telomerase activity is described in which the duplex scorpion primer is used to provide an intramolecular probing mechanism for specific detection of telomerase activity. Using this method, linearity from 10 to 10(4) cells expressing telomerase activity could be obtained (R-2 = 0.994). The requirement of post-PCR steps is thus obviated.

引用

页码：891 / 895

页数：5

共 12 条

[1]

BERANCCHI S, 2001, NUCLEIC ACIDS RES, V29, pE62

[2] Real-time quantitative analysis of telomerase activity in breast tumor specimens using a highly specific and sensitive fluorescent-based assay [J].