Genome-wide microRNA profiles identify miR-378 as a serum biomarker for early detection of gastric cancer

被引：225

作者：

Liu, Hanshao ^{[1
,2
]}

Zhu, Lin ^{[3
]}

Liu, Bingya ^{[4
]}

Yang, Liu ^{[4
]}

Meng, Xianxin ^{[1
]}

Zhang, Wei ^{[1
]}

Ma, Yueyun ^{[3
]}

Xiao, Huasheng ^{[1
,2
]}

机构：

[1] Natl Engn Ctr Biochip Shanghai, Shanghai 201203, Peoples R China

[2] Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Dis & Hlth Genom, Shanghai 201203, Peoples R China

[3] Fourth Mil Med Univ, PLA, Xijing Hosp, Ctr Clin Lab Med, Xian 710032, Peoples R China

[4] Shanghai Jiao Tong Univ, Shanghai Rui Jin Hosp, Sch Med, Shanghai 200025, Peoples R China

来源：

CANCER LETTERS | 2012年 / 316卷 / 02期

关键词：

Gastric cancer; Genome-wide microRNA expression profile; Serum microRNA; Noninvasive biomarker; CIRCULATING MICRORNAS; CAENORHABDITIS-ELEGANS; EXPRESSION; PCR; DIAGNOSIS; CA-72-4; CA-19-9; CEA;

D O I：

10.1016/j.canlet.2011.10.034

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Recent studies demonstrated that in several human malignancies aberrant expression profiles of circulating microRNAs (miRNAs) anticipate great cancer diagnostic potential. Here we showed that serum miR-378 could serve as a novel noninvasive biomarker in gastric cancer (GC) detection. Genome-wide miRNA expression profiles followed with Real-Time quantitative RT-PCR (qRT-PCR) assays revealed that miR-187*, miR-371-5p and miR-378 were significantly elevated in GC patients. Further validation indicated that miR-378 alone could yields a ROC curve area of 0.861 with 87.5% sensitivity and 70.73% specificity in discriminating GC patients from healthy controls. Collectively, these data support our contention that serum miR-378 has strong potential as a novel noninvasive biomarker in gastric cancer detection. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：196 / 203

页数：8

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