Four genes located on a SSC2 meat quality QTL region are associated with different meat quality traits in Landrace Chinese-European crossbred population

被引:10
作者
Cepica, S. [1 ]
Ovilo, C. [2 ]
Masopust, M. [1 ]
Knoll, A. [1 ,3 ]
Fernandez, A. [2 ]
Lopez, A. [2 ]
Rohrer, G. A. [4 ]
Nonneman, D. [4 ]
机构
[1] AS CR, Inst Anim Physiol & Genet, Libechov 27721, Czech Republic
[2] Inst Nacl Invest & Tecnol Agr & Alimentaria, Madrid 28040, Spain
[3] Mendel Univ Brno, Brno 61300, Czech Republic
[4] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA
关键词
association study; complement factor D (adipsin); insulin receptor; meat quality; pig; porcine chromosome 2; resistin; ubiquitin-like; 5; GENOME; PIG; METABOLISM; VARIANTS; FATNESS; BEACON; UBL5; MAP;
D O I
10.1111/j.1365-2052.2011.02252.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Several quantitative trait loci (QTL) for different meat quality traits have been localized on the q arm of porcine chromosome 2 at position 5578 cM. Association analyses were performed in a commercial Landrace X Chinese-European (LCE) crossbred population (n = 446) slaughtered at approximately 127 kg and an average age of 198 days with records for performance (growth, fat and meat accretion) and meat quality [intramuscular fat (IMF), Minolta L*, Minolta a*, Minolta b* and pH at 45 m]. Polymorphisms within positional candidate genes cloned from homologous regions on human chromosome 19, ubiquitin-like 5 (UBL5 AM950288:g.566G>A), resistin (RETN AM157180:g.1473A>G causing substitution p.Ala36Thr), insulin receptor (INSR AM950289:g.589T>C) and complement factor D (adipsin) (CFD AM950287:g. 306C>T) were located at positions 62.1, 64.0, 68.0 and 70.7 cM respectively on the current USDA USMARC map of porcine chromosome 2 and had the following allele frequencies in the LCE: UBL5 566G 0.57; RETN 1473G 0.84; INSR 589C 0.70; and CFD 306C 0.73. The effects of alleles within the candidate genes on the recorded traits were estimated using an animal model. Significant effects (P < 0.05) were found for pH45 in m. semimembranosus (m. sm.) (UBL5), IMF (RETN) and Minolta L* (RETN, CFD). Differences between phenotypic means of homozygotes at UBL5, RETN and either RETN or CFD explained 0.34 SD for pH45 in m. sm., 0.47 SD for IMF and 0.68 SD for Minolta L* respectively. Suggestive effects (P < 0.10) on IMF (UBL5, CFD), Minolta a* (INSR, CFD) and Minolta b* (INSR) were also observed. Our results support the localization of further QTL for meat quality traits in this region and suggest that there are several genes affecting different meat quality traits.
引用
收藏
页码:333 / 336
页数:4
相关论文
共 18 条
[1]   Association of genetic variation within UBL5 with phenotypes of metabolic syndrome [J].
Bozaoglu, Kiymet ;
Curran, Joanne E. ;
Elliott, Kate S. ;
Walder, Ken R. ;
Dyer, Thomas D. ;
Rainwater, David L. ;
Vandeberg, John L. ;
Comuzzie, Anthony G. ;
Collier, Greg R. ;
Zimmet, Paul ;
Maccluer, Jean W. ;
Jowett, Jeremy B. ;
Blangero, John .
HUMAN BIOLOGY, 2006, 78 (02) :147-159
[2]   Role of brain insulin receptor in control of body weight and reproduction [J].
Brüning, JC ;
Gautam, D ;
Burks, DJ ;
Gillette, J ;
Schubert, M ;
Orban, PC ;
Klein, R ;
Krone, W ;
Müller-Wieland, D ;
Kahn, CR .
SCIENCE, 2000, 289 (5487) :2122-2125
[3]   Partial cloning, cytogenetic and linkage mapping of the porcine resistin (RSTN) gene [J].
Cepica, S ;
Rohrer, GA ;
Masopust, M ;
Kubickova, S ;
Musilova, P ;
Rubes, J .
ANIMAL GENETICS, 2002, 33 (05) :381-383
[4]   Critical review of acylation-stimulating protein physiology in humans and rodents [J].
Cianflone, K ;
Xia, ZN ;
Chen, LY .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2003, 1609 (02) :127-143
[5]   Association studies on the porcine RETN, UCP1, UCP3 and ADRB3 genes polymorphism with fatness traits [J].
Cieslak, Jakub ;
Nowacka-Woszuk, Joanna ;
Bartz, Magdalena ;
Fijak-Nowak, Honorata ;
Grzes, Maria ;
Szydiowski, Maciej ;
Switonski, Marek .
MEAT SCIENCE, 2009, 83 (03) :551-554
[6]   Beacon - A novel gene involved in the regulation of energy balance [J].
Collier, GR ;
McMillan, JS ;
Windmill, K ;
Walder, K ;
Tenne-Brown, J ;
de Silva, A ;
Trevaskis, J ;
Jones, S ;
Morton, GJ ;
Lee, S ;
Augert, G ;
Civitarese, A ;
Zimmet, PZ .
DIABETES, 2000, 49 (11) :1766-1771
[7]  
Du FX, 2007, INT J BIOL SCI, V3, P166
[8]   Genome-wide identification of quantitative trait loci for pork temperature, pH decline, and glycolytic potential in a large-scale White Duroc x Chinese Erhualian resource population [J].
Duan, Y. -Y. ;
Ma, J. -W. ;
Yuan, F. ;
Huang, L. -B. ;
Yang, K. -X. ;
Xie, J. -P. ;
Wu, G. -Z. ;
Huang, L. -S. .
JOURNAL OF ANIMAL SCIENCE, 2009, 87 (01) :9-16
[9]   Influence of intramuscular fat content on the quality of pig meat -: 1.: Composition of the lipid fraction and sensory characteristics of m. longissimus lumborum [J].
Fernandez, X ;
Monin, G ;
Talmant, A ;
Mourot, J ;
Lebret, B .
MEAT SCIENCE, 1999, 53 (01) :59-65
[10]  
HILL W G, 1968, Theoretical and Applied Genetics, V38, P226, DOI 10.1007/BF01245622