Prospects for drug screening using the reverse two-hybrid system

被引：67

作者：

Vidal, M ^{[1
]}

Endoh, H ^{[1
]}

机构：

[1] Massachusetts Gen Hosp, Ctr Canc, Charlestown, MA 02129 USA

来源：

TRENDS IN BIOTECHNOLOGY | 1999年 / 17卷 / 09期

关键词：

D O I：

10.1016/S0167-7799(99)01338-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rational drug-screening strategies have been limited by the number of available protein targets, The fields of genomics and functional genomics are now merging into 'chemical genomics' approaches, in which large numbers of potential target proteins can be used in standardized high-throughput drug-screening assays, Because protein-protein interactions are critical to most biological processes and can be tested in standardized assays, they may represent optimal targets in the chemical-genomics era. The reverse two-hybrid system appears to have several properties that would be critical for the success of this approach.

引用

页码：374 / 381

页数：10

共 57 条

[1] DIRECT SELECTIVE TECHNIQUES FOR ISOLATION OF PYRIMIDINE AUXOTROPHS IN YEAST [J].

BACH, ML ;

LACROUTE, F .

MOLECULAR AND GENERAL GENETICS, 1972, 115 (02) :126-&

[2] YEAST MULTIDRUG-RESISTANCE - THE PDR NETWORK [J].

BALZI, E ;

GOFFEAU, A .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1995, 27 (01) :71-76

[3] A protein linkage map of Escherichia coli bacteriophage T7 [J].

Bartel, PL ;

Roecklein, JA ;

SenGupta, D ;

Fields, S .

NATURE GENETICS, 1996, 12 (01) :72-77

[4] Small molecule-dependent genetic selection in stochastic nanodroplets as a means of detecting protein-ligand interactions on a large scale [J].

Borchardt, A ;

Liberles, SD ;

Biggar, SR ;

Crabtree, GR ;

Schreiber, SL .

CHEMISTRY & BIOLOGY, 1997, 4 (12) :961-968

[5] Dominant-negative p53 mutations selected in yeast hit cancer hot spots [J].

Brachmann, RK ;

Vidal, M ;

Boeke, JD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (09) :4091-4095

[6] NEW INTRACELLULAR TARGETS FOR THERAPEUTIC DRUG DESIGN [J].

BRUGGE, JS .

SCIENCE, 1993, 260 (5110) :918-919

[7] Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii [J].

Bult, CJ ;

White, O ;

Olsen, GJ ;

Zhou, LX ;

Fleischmann, RD ;

Sutton, GG ;

Blake, JA ;

FitzGerald, LM ;

Clayton, RA ;

Gocayne, JD ;

Kerlavage, AR ;

Dougherty, BA ;

Tomb, JF ;

Adams, MD ;

Reich, CI ;

Overbeek, R ;

Kirkness, EF ;

Weinstock, KG ;

Merrick, JM ;

Glodek, A ;

Scott, JL ;

Geoghagen, NSM ;

Weidman, JF ;

Fuhrmann, JL ;

Nguyen, D ;

Utterback, TR ;

Kelley, JM ;

Peterson, JD ;

Sadow, PW ;

Hanna, MC ;

Cotton, MD ;

Roberts, KM ;

Hurst, MA ;

Kaine, BP ;

Borodovsky, M ;

Klenk, HP ;

Fraser, CM ;

Smith, HO ;

Woese, CR ;

Venter, JC .

SCIENCE, 1996, 273 (5278) :1058-1073

[8] Transdominant genetic analysis of a growth control pathway [J].

Caponigro, G ;

Abedi, MR ;

Hurlburt, AP ;

Maxfield, A ;

Judd, W ;

Kamb, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (13) :7508-7513

[9] Genetic selection of peptide aptamers that recognize and inhibit cyclin-dependent kinase 2 [J].

Colas, P ;

Cohen, B ;

Jessen, T ;

Grishina, I ;

McCoy, J ;

Brent, R .

NATURE, 1996, 380 (6574) :548-550

[10] THE RETINOBLASTOMA PROTEIN ASSOCIATES WITH THE PROTEIN PHOSPHATASE TYPE-1 CATALYTIC SUBUNIT [J].

DURFEE, T ;

BECHERER, K ;

CHEN, PL ;

YEH, SH ;

YANG, YZ ;

KILBURN, AE ;

LEE, WH ;

ELLEDGE, SJ .

GENES & DEVELOPMENT, 1993, 7 (04) :555-569

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