Serotype and apx genotype profiles of Actinobacillus pleuropneumoniae field isolates in Korea

被引：28

作者：

Min, K ^{[1
]}

Chae, C ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Vet Med, Dept Vet Pathol, Suwon 441744, Kyounggi Do, South Korea

来源：

VETERINARY RECORD | 1999年 / 145卷 / 09期

关键词：

D O I：

10.1136/vr.145.9.251

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A total of Inn field isolates of Actinobacillus pleuropneumoniae isolated from lung tissues of pigs with severe pleuropneumonia were serotyped by slide agglutination and precipitation tests. Polymerase chain reactions for opxICA, apxIICA, apxIIICA, apxIBD and opxIIIBD genes were used to determine their genotype prevalence. Serotypes 2 (56 isolates), 5 (28 isolates) and 6 (11 isolates) were the most common; only two isolates belonged to serotype 7, and three were untyped. Among the 97 isolates identified by serotype, 70 had the same apr genes as their respective serotype reference strains, but 27 did not have any of the apx genes present in the corresponding serotype reference strain. Among these 27 isolates, 10 were serotype 2, 12 were serotype 5, three were serotype 6 and two were serotype 7.

引用

页码：251 / 254

页数：4

共 24 条

[1] ISOLATION AND MOLECULAR CHARACTERIZATION OF SPONTANEOUSLY OCCURRING CYTOLYSIN-NEGATIVE MUTANTS OF ACTINOBACILLUS-PLEUROPNEUMONIAE SEROTYPE-7 [J].

ANDERSON, C ;

POTTER, AA ;

GERLACH, GF .

INFECTION AND IMMUNITY, 1991, 59 (11) :4110-4116

[2] RTX TOXIN GENOTYPES AND PHENOTYPES IN ACTINOBACILLUS-PLEUROPNEUMONIAE FIELD STRAINS [J].

BECK, M ;

VANDENBOSCH, JF ;

JONGENELEN, IMCA ;

LOEFFEN, PLW ;

NIELSEN, R ;

NICOLET, J ;

FREY, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (11) :2749-2754

[3]

BERTRAM TA, 1990, CAN J VET RES, V54, pS53

[4] HUMORAL ANTIBODY-RESPONSE AND PROTECTIVE IMMUNITY IN SWINE FOLLOWING IMMUNIZATION WITH THE 104-KILODALTON HEMOLYSIN OF ACTINOBACILLUS-PLEUROPNEUMONIAE [J].

DEVENISH, J ;

ROSENDAL, S ;

BOSSE, JT .

INFECTION AND IMMUNITY, 1990, 58 (12) :3829-3832

[5] SEROTYPING AND QUANTITATIVE-DETERMINATION OF IN-VITRO ANTIBIOTIC SUSCEPTIBILITY OF ACTINOBACILLUS-PLEUROPNEUMONIAE STRAINS ISOLATED IN BELGIUM (JULY 1991 - AUGUST 1992) [J].

DOM, P ;

HOMMEZ, J ;

CASTRYCK, F ;

DEVRIESE, LA ;

HAESEBROUCK, F .

VETERINARY QUARTERLY, 1994, 16 (01) :10-13

[6]

Fales W H, 1989, J Vet Diagn Invest, V1, P16

[7] VIRULENCE IN ACTINOBACILLUS-PLEUROPNEUMONIAE AND RTX TOXINS [J].

FREY, J .

TRENDS IN MICROBIOLOGY, 1995, 3 (07) :257-261

[8] DEVELOPMENT OF AN EFFICIENT PCR METHOD FOR TOXIN TYPING OF ACTINOBACILLUS-PLEUROPNEUMONIAE STRAINS [J].

FREY, J ;

BECK, M ;

VANDENBOSCH, JF ;

SEGERS, RPAM ;

NICOLET, J .

MOLECULAR AND CELLULAR PROBES, 1995, 9 (04) :277-282

[9] SEROLOGICAL CHARACTERIZATION AND ANTIMICROBIAL SUSCEPTIBILITY OF ACTINOBACILLUS-PLEUROPNEUMONIAE STRAINS ISOLATED FROM PIGS IN SPAIN [J].

GUTIERREZ, CB ;

BARBOSA, JIR ;

TASCON, RI ;

COSTA, L ;

RIERA, P ;

FERRI, EFR .

VETERINARY RECORD, 1995, 137 (03) :62-64

[10] SLIDE PRECIPITATION - A SIMPLE METHOD TO TYPE ACTINOBACILLUS-(HAEMOPHILUS)-PLEUROPNEUMONIAE [J].

HOMMEZ, J ;

DEVRIESE, LA ;

CASTRYCK, F ;

CASSIMON, P .

VETERINARY MICROBIOLOGY, 1990, 24 (02) :123-126

← 1 2 3 →