IDENTIFICATION AND ANALYSIS OF TRNAS THAT ARE DEGRADED IN SACCHAROMYCES CEREVISIAE DUE TO LACK OF MODIFICATIONS

被引：15

作者：

Chernyakov, Irina ^{[1
]}

Baker, Melanie A. ^{[1
]}

Grayhack, Elizabeth J. ^{[1
]}

Phizicky, Eric M. ^{[1
]}

机构：

[1] Univ Rochester, Dept Biochem & Biophys, Sch Med, Rochester, NY 14627 USA

来源：

RNA TURNOVER IN EUKARYOTES: ANALYSIS OF SPECIALIZED AND QUALITY CONTROL RNA DECAY PATHWAYS | 2008年 / 449卷

关键词：

D O I：

10.1016/S0076-6879(08)02411-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mounting evidence shows that tRNA modifications play crucial roles in the maintenance of wild-type levels of several tRNA species. This chapter describes a generalized framework in which to study tRNA turnover in the yeast Saccharomyces cerevisiae as a consequence of a defect in tRNA modification status. It describes several approaches for the identification of tRNA species that are reduced as a consequence of a modification defect, methods for analysis of the rate of tRNA loss and analysis of its aminoacylation, and methods for initial characterization of tRNA turnover. These approaches have been used successfully for several modification defects that result in tRNA turnover.

引用

页码：221 / 237

页数：17

共 25 条

[1] Rapid tRNA decay can result from lack of nonessential modifications [J].

Alexandrov, A ;

Chernyakov, I ;

Gu, WF ;

Hiley, SL ;

Hughes, TR ;

Grayhack, EJ ;

Phizicky, EM .

MOLECULAR CELL, 2006, 21 (01) :87-96

[2] The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA [J].

Anderson, J ;

Phan, L ;

Cuesta, R ;

Carlson, BA ;

Pak, M ;

Asano, K ;

Björk, GR ;

Tamame, M ;

Hinnebusch, AG .

GENES & DEVELOPMENT, 1998, 12 (23) :3650-3662

[3] The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability [J].

Copela, LA ;

Chakshusmathi, G ;

Sherrer, RL ;

Wolin, SL .

RNA, 2006, 12 (04) :644-654

[4] PROBING STRUCTURAL DIFFERENCES BETWEEN NATIVE AND IN-VITRO TRANSCRIBED ESCHERICHIA-COLI VALINE TRANSFER-RNA - EVIDENCE FOR STABLE BASE MODIFICATION-DEPENDENT CONFORMERS [J].

DERRICK, WB ;

HOROWITZ, J .

NUCLEIC ACIDS RESEARCH, 1993, 21 (21) :4948-4953

[5] Selective charging of tRNA isoacceptors induced by amino-acid starvation [J].

Dittmar, KA ;

Sorensen, MA ;

Elf, J ;

Ehrenberg, M ;

Pan, T .

EMBO REPORTS, 2005, 6 (02) :151-157

[6] Depletion of Saccharomyces cerevisiae tRNAHis guanylyltransferase Thg1p leads to uncharged tRNAHis with additional m5C [J].

Gu, WF ;

Hurto, RL ;

Hopper, AK ;

Grayhack, EJ ;

Phizicky, EM .

MOLECULAR AND CELLULAR BIOLOGY, 2005, 25 (18) :8191-8201

[7] STRUCTURE OF AN UNMODIFIED TRANSFER-RNA MOLECULE [J].

HALL, KB ;

SAMPSON, JR ;

UHLENBECK, OC ;

REDFIELD, AG .

BIOCHEMISTRY, 1989, 28 (14) :5794-5801

[8] Detection and discovery of RNA modifications using microarrays [J].

Hiley, SL ;

Jackman, J ;

Babak, T ;

Trochesset, M ;

Morris, QD ;

Phizicky, E ;

Hughes, TR .

NUCLEIC ACIDS RESEARCH, 2005, 33 (01) :e2

[9] Microarray analysis of RNA processing and modification [J].

Hughes, Timothy R. ;

Hiley, Shawna L. ;

Saltzman, Arneet L. ;

Babak, Tomas ;

Blencowe, Benjamin J. .

DNA MICROARRAYS PART A: ARRAY PLATFORMS AND WET-BENCH PROTOCOLS, 2006, 410 :300-316

[10] MODE OF ACTION OF THIOLUTIN, AN INHIBITOR OF MACROMOLECULAR SYNTHESIS IN SACCHAROMYCES-CEREVISIAE [J].

JIMENEZ, A ;

TIPPER, DJ ;

DAVIES, J .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1973, 3 (06) :729-738

← 1 2 3 →