Short gamete co-incubation during in vitro fertilization decreases the fertilization rate and does not improve embryo quality:: a prospective auto controlled study

被引：32

作者：

Barraud-Lange, V. ^{[1
]}

Sifer, C. ^{[1
]}

Pocate, K. ^{[1
]}

Ziyyat, A. ^{[1
]}

Martin-Pont, B. ^{[1
]}

Porcher, R. ^{[2
]}

Hugues, J. N. ^{[3
]}

Wolf, J. P. ^{[1
]}

机构：

[1] Hop Jean Verdier, Serv Histol Embryol Cytogenet, F-93140 Bondy, France

[2] CHU St Louis, Serv Biostat & Informat Med, F-75475 Paris 10, France

[3] Hop Jean Verdier, Serv Med Reprod, F-93140 Bondy, France

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2008年 / 25卷 / 07期

关键词：

fertilization rate; embryo quality; short gamete insemination; human IVF;

D O I：

10.1007/s10815-008-9240-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose Evaluate the effect of short gamete incubation on fertilization rate and embryo quality. Methods A prospective study has been performed. Two thousand five hundred and forty seven sibling oocytes from 240 couples undergoing IVF attempts were allocated to a short (1 h) or a standard (18 h) insemination procedure. Diploid fertilization rate (two pronuclei, 2PN), polyspermy (> 2PN) and embryo quality were compared. Results The fertilization rate was statistically lower in the short insemination group compared to the standard insemination one (64.9% and 70.1%; P=0.039), with a similar polyspermy rate observed between the two groups. A slight, but non significant, increase was observed concerning good embryo quality rate in the short insemination group when compared to the standard insemination, both at day 2 (60.1 vs. 58.1%; P=0.06) and day 3 (53.2 vs. 48.5%; P=0.22). Conclusion This new study highlights that a 1 h gamete exposure decreases the fertilization rate and does not improve embryo quality compared with a standard 18 h insemination procedure.

引用

页码：305 / 310

页数：6