Characterization of a chicken monoclonal antibody that recognizes the apical complex of Eimeria acervulina sporozoites and partially inhibits sporozoite invasion CD8(+) T lymphocytes in vitro

被引：29

作者：

Sasai, K ^{[1
]}

Lillehoj, HS ^{[1
]}

Matsuda, H ^{[1
]}

Wergin, WP ^{[1
]}

机构：

[1] USDA,ARS,INST LIVESTOCK & POULTRY SCI,PARASITE IMMUNOBIOL LAB,BELTSVILLE,MD 20705

来源：

JOURNAL OF PARASITOLOGY | 1996年 / 82卷 / 01期

关键词：

D O I：

10.2307/3284120

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A Stable chicken hybridoma secreting a monoclonal antibody (mAb) that detects the apical complex of Eimeria acervulina sporozoites has been developed by fusing a thymidine kinase (TK)-deficient chicken myeloma with spleen cells from chickens immunized with sporozoite antigen. The mAb, designated as 6D-12-G10, recognized the apical complex of a sporozoite of 20-21 kDa molecular mass on western blots. Immunoelectron microscopic examination revealed that mAb 6D-12-G10 stained the conoid antigen. Furthermore, mAb 6D-12-G10 inhibited the invasion of sporozoites into CD8(+) T cells in vitro. These results suggest that the conoid may play an important role in the recognition and invasion of host cells by Eimeria sporozoites.

引用

页码：82 / 87

页数：6

共 27 条

[11] FUNCTIONAL AND BIOCHEMICAL CHARACTERIZATIONS OF AVIAN LYMPHOCYTE-T ANTIGENS IDENTIFIED BY MONOCLONAL-ANTIBODIES [J].

LILLEHOJ, HS ;

LILLEHOJ, EP ;

WEINSTOCK, D ;

SCHAT, KA .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (12) :2059-2065

[12] CD8+ T-CELL-COCCIDIA INTERACTIONS [J].

LILLEHOJ, HS ;

TROUT, JM .

PARASITOLOGY TODAY, 1994, 10 (01) :10-14

[13] COCCIDIA - A REVIEW OF RECENT ADVANCES ON IMMUNITY AND VACCINE DEVELOPMENT [J].

LILLEHOJ, HS ;

TROUT, JM .

AVIAN PATHOLOGY, 1993, 22 (01) :3-31

[14] DEVELOPMENT AND CHARACTERIZATION OF CHICKEN-CHICKEN B-CELL HYBRIDOMAS SECRETING MONOCLONAL-ANTIBODIES THAT DETECT SPOROZOITE AND MEROZOITE ANTIGENS OF EIMERIA [J].

LILLEHOJ, HS ;

SASAI, K ;

MATSUDA, H .

POULTRY SCIENCE, 1994, 73 (11) :1685-1693

[15]

LILLEHOJ HS, 1986, AVIAN DIS, V31, P112

[16] A NEW CELL-LINE FOR THE PRODUCTION OF CHICKEN MONOCLONAL-ANTIBODY BY HYBRIDOMA TECHNOLOGY [J].

NISHINAKA, S ;

SUZUKI, T ;

MATSUDA, H ;

MURATA, M .

JOURNAL OF IMMUNOLOGICAL METHODS, 1991, 139 (02) :217-222

[17] RHOPTRY ORGANELLES OF APICOMPLEXAN PARASITES [J].

PERKINS, ME .

PARASITOLOGY TODAY, 1992, 8 (01) :28-32

[18] THE EFFECT OF IMMUNITY ON THE EARLY EVENTS IN THE LIFE-CYCLE OF EIMERIA-TENELLA IN THE CECAL MUCOSA OF THE CHICKEN [J].

ROSE, ME ;

LAWN, AM ;

MILLARD, BJ .

PARASITOLOGY, 1984, 88 (APR) :199-210

[19]

RUSSELL DG, 1984, J CELL SCI, V65, P193

[20] METHODS IN COCCIDIOSIS RESEARCH - SEPARATION OF OOCYSTS FROM FECES [J].

RYLEY, JF ;

MEADE, R ;

HAZELHURST, J ;

ROBINSON, TE .

PARASITOLOGY, 1976, 73 (DEC) :311-326

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