Prediction of dynamic metabolic behavior of Pediococcus pentosaceus producing lactic acid from lignocellulosic sugars

被引：5

作者：

Adler, Philipp ^{[1
]}

Song, Hyun-Seob ^{[2
]}

Kaestner, Katharina ^{[1
]}

Ramkrishna, Doraiswami ^{[2
]}

Kunz, Benno ^{[1
]}

机构：

[1] Univ Bonn, Inst Nutr & Food Sci, Div Food Technol & Biotechnol, D-53117 Bonn, Germany

[2] Purdue Univ, Sch Chem Engn, W Lafayette, IN 47907 USA

来源：

BIOTECHNOLOGY PROGRESS | 2012年 / 28卷 / 03期

关键词：

lactic acid; hybrid cybernetic model; elementary mode; lignocellulose; Pediococcus pentosaceus; ELEMENTARY FLUX MODES; CYBERNETIC MODELS; MICROBIAL SYSTEM; LACTOCOCCUS-LACTIS; GROWTH; GLUCOSE; STRAIN; XYLOSE; BATCH; COLI;

D O I：

10.1002/btpr.1521

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A dynamic metabolic model is presented for Pediococcus pentosaceus producing lactic acid from lignocellulose-derived mixed sugars including glucose, mannose, galactose, arabinose, and xylose. Depending on the pairs of mixed sugars, P. pentosaceus exhibits diverse (i.e., sequential, simultaneous or mixed) consumption patterns. This regulatory behavior of P. pentosaceus is portrayed using the hybrid cybernetic model (HCM) framework which views elementary modes of the network as metabolic options dynamically modulated. Comprehensive data are collected for model identification and validation through fermentation experiments involving single substrates and various combinations of mixed sugars. Most sugars are metabolized rather sequentially while co-consumption of galactose and arabinose is observed. It is demonstrated that the developed HCM successfully predicts mixed sugar data based on the parameters identified mostly from single substrate data only. Further, we discuss the potential of HCMs as a tool for predicting intracellular flux distribution with comparison with flux balance analysis. (C) 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012

引用

页码：623 / 635

页数：13

共 38 条

[11] GENERAL PHYSIOLOGICAL CONSIDERATIONS OF CATABOLITE REPRESSION IN PEDIOCOCCUS PENTOSACEUS [J].

DOBROGOSZ, WJ ;

DEMOSS, RD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1963, 77 (04) :639-&

[12]

GROHMANN K, 1994, ACS SYM SER, V566, P372

[13] Factors affecting the fermentative lactic acid production from renewable resources [J].

Hofvendahl, K ;

Hahn-Hägerdal, B .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 26 (2-4) :87-107

[14] Fermentative production of lactic acid from biomass: an overview on process developments and future perspectives [J].

John, Rojan P. ;

Nampoothiri, K. Madhavan ;

Pandey, Ashok .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 74 (03) :524-534

[15] A Hybrid Model of Anaerobic E. coli GJT001: Combination of Elementary Flux Modes and Cybernetic Variables [J].

Kim, Jin Il ;

Varner, Jeffery D. ;

Ramkrishna, Doraiswami .

BIOTECHNOLOGY PROGRESS, 2008, 24 (05) :993-1006

[16] INVESTIGATION OF BACTERIAL-GROWTH ON MIXED SUBSTRATES - EXPERIMENTAL EVALUATION OF CYBERNETIC MODELS [J].

KOMPALA, DS ;

RAMKRISHNA, D ;

JANSEN, NB ;

TSAO, GT .

BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (07) :1044-1055

[17] Comparative genomics of the lactic acid bacteria [J].

Makarova, K. ;

Slesarev, A. ;

Wolf, Y. ;

Sorokin, A. ;

Mirkin, B. ;

Koonin, E. ;

Pavlov, A. ;

Pavlova, N. ;

Karamychev, V. ;

Polouchine, N. ;

Shakhova, V. ;

Grigoriev, I. ;

Lou, Y. ;

Rohksar, D. ;

Lucas, S. ;

Huang, K. ;

Goodstein, D. M. ;

Hawkins, T. ;

Plengvidhya, V. ;

Welker, D. ;

Hughes, J. ;

Goh, Y. ;

Benson, A. ;

Baldwin, K. ;

Lee, J. -H. ;

Diaz-Muniz, I. ;

Dosti, B. ;

Smeianov, V. ;

Wechter, W. ;

Barabote, R. ;

Lorca, G. ;

Altermann, E. ;

Barrangou, R. ;

Ganesan, B. ;

Xie, Y. ;

Rawsthorne, H. ;

Tamir, D. ;

Parker, C. ;

Breidt, F. ;

Broadbent, J. ;

Hutkins, R. ;

O'Sullivan, D. ;

Steele, J. ;

Unlu, G. ;

Saier, M. ;

Klaenhammer, T. ;

Richardson, P. ;

Kozyavkin, S. ;

Weimer, B. ;

Mills, D. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (42) :15611-15616

[18] STRUCTURED MODELING OF A MICROBIAL SYSTEM .1. A THEORETICAL-STUDY OF LACTIC-ACID FERMENTATION [J].

NIELSEN, J ;

NIKOLAJSEN, K ;

VILLADSEN, J .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (01) :1-10

[19] STRUCTURED MODELING OF A MICROBIAL SYSTEM .2. EXPERIMENTAL-VERIFICATION OF A STRUCTURED LACTIC-ACID FERMENTATION MODEL [J].

NIELSEN, J ;

NIKOLAJSEN, K ;

VILLADSEN, J .

BIOTECHNOLOGY AND BIOENGINEERING, 1991, 38 (01) :11-23

[20]

Nielsen J., 2003, BIOREACTION ENG PRIN, V2nd

← 1 2 3 4 →