Evaluation of microarray surfaces and arraying parameters for autoantibody profiling

被引：40

作者：

Balboni, Imelda ^{[1
,2
]}

Limb, Cindy ^{[1
]}

Tenenbaum, Jessica D.

Utz, Paul J. ^{[1
,3
]}

机构：

[1] Stanford Univ, Dept Med, Div Rheumatol & Immunol, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Pediat, Div Rheumatol, Stanford, CA 94305 USA

[3] Stanford Univ, Dept Med, Stanford, CA 94305 USA

来源：

PROTEOMICS | 2008年 / 8卷 / 17期

关键词：

autoantibody profiling; autoantigens; autoimmune disease; multiplexed proteomics technology; protein microarrays;

D O I：

10.1002/pmic.200800146

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Autoantigen microarrays are being used increasingly to study autoimmunity. Significant variation has been observed when comparing microarray surfaces, printing methods, and probing conditions. In the present study, 24 surfaces and several arraying parameters were analyzed using >500 feature autoantigen microarrays printed with quill pins. A small subset of slides, including FAST (R), PATH (R), and SuperEpoxy2, performed well while maintaining the sensitivity and specificity of autoantigen microarrays previously demonstrated by our laboratory. By optimizing the major variables in our autoantigen microarray platform, subtle differences in serum samples can be identified that will shed light on disease pathogenesis.

引用

页码：3443 / 3449

页数：7

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