Crystallization and preliminary X-ray studies of the basic lectin from the seeds of Artocarpus hirsuta

The basic lectin from Artocarpus hirsuta specific towards methyl ol-galactose has been purified and crystallized using the hanging-drop vapour-diffusion method with ammonium sulfate as precipitant. Three different crystal forms, orthorhombic I, orthorhombic II and hexagonal, were grown under the same crystallization conditions. The orthorhombic forms belonged to space group P2(1)2(1)2(1) with unit-cell dimensions a = 92.9, b = 99.8, c = 166.2 Angstrom and a = 89.9, b = 121.9, c = 131.6 Angstrom, respectively. The unit-cell dimensions of the hexagonal form were a = b = 84.1 and c = 271.7 Angstrom and the space group was P6(1)22.