Improvement of spermatogenesis in adult cryptorchid rat testis by intratesticular infusion of lactate

被引:75
作者
Courtens, JL [1 ]
Plöen, L
机构
[1] INRA, Dept Reprod Physiol Mammiferes Domest, F-37380 Nouzilly, France
[2] Swedish Univ Agr Sci, Ctr Reprod Sci, Dept Anat & Histol, S-75007 Uppsala, Sweden
关键词
D O I
10.1095/biolreprod61.1.154
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In order to test the hypothesis that a lack of energy could be a cause of germ cell death at high temperatures, cryptorchid rats testes were infused with lactate, delivered by osmotic pumps over 3-15 days. In cryptorchid testes, the spermatids and spermatocytes were lost between 3 and 8 days. In cryptorchid testes supplemented with lactate, elongated spermatids persisted in a few seminiferous tubules at Day 15. Elimination of round spermatids occurred progressively between 3 and 15 days, mostly at stage VIII. The loss of spermatocytes increased after 8 days, and 30% of seminiferous tubules still contained meiotic or meiotic plus spermiogenetic cells at Day 15. After 8 days, the chromatin of step 8 round spermatids was abnormal and nuclear elongation did not commence. The Sertoli cell cytoplasm that was retracted toward the basal compartment of the seminiferous epithelium could not hold the germ cells of the adluminal compartment. Therefore, attachment of germ cells to Sertoli cells and the supply of lactate seem necessary for the development of germ cells at high temperatures. The improvement in spermatogenesis in cryptorchid supplemented testes for several days is a new finding.
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收藏
页码:154 / 161
页数:8
相关论文
共 54 条
  • [21] ANTISPERMATOGENIC EFFECTS OF 5-THIO-D-GLUCOSE IN MALE RATS
    HOMM, RE
    RUSTICUS, C
    HAHN, DW
    [J]. BIOLOGY OF REPRODUCTION, 1977, 17 (05) : 697 - 700
  • [22] JUTTE NHP, 1982, THESIS ERASMUS U ROT
  • [23] JUTTE NHPM, 1981, J REPROD FERTIL, V62, P399, DOI 10.1530/jrf.0.0620399
  • [24] KALLERHOFF M, 1993, HELV CHIR ACTA, V60, P371
  • [25] RECOVERY OF TESTICULAR FUNCTIONS AFTER SURGICAL-TREATMENT OF EXPERIMENTAL CRYPTORCHIDISM IN THE RAT
    KARPE, B
    PLOEN, L
    HAGENAS, L
    RITZEN, EM
    [J]. INTERNATIONAL JOURNAL OF ANDROLOGY, 1981, 4 (02): : 145 - 160
  • [26] DISTRIBUTION OF ACTIN IN SPERMATIDS AND ADJACENT SERTOLI-CELL REGIONS OF THE RAT
    MASRI, BA
    RUSSELL, LD
    VOGL, AW
    [J]. ANATOMICAL RECORD, 1987, 218 (01): : 20 - 26
  • [27] Regulation by pH of the alternative splicing of the stem cell factor pre-mRNA in the testis
    Mauduit, C
    Chatelain, G
    Magre, S
    Brun, G
    Benhamed, M
    Michel, D
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (02) : 770 - 775
  • [28] McKinnell C, 1997, INT J ANDROL, V20, P171
  • [29] Neonatal exposure of rats to recombinant follicle stimulating hormone increases adult sertoli and spermatogenic cell numbers
    Meachem, SJ
    McLachlan, RI
    deKretser, DM
    Robertson, DM
    Wreford, NG
    [J]. BIOLOGY OF REPRODUCTION, 1996, 54 (01) : 36 - 44
  • [30] Mizuno K, 1996, CELL DEATH DIFFER, V3, P119