A non-isotopic assay for histone deacetylase activity

被引：94

作者：

Hoffmann, K

Brosch, G

Loidl, P

Jung, M

机构：

[1] Univ Munster, Dept Pharmaceut Chem, D-48149 Munster, Germany

[2] Univ Innsbruck, Sch Med, Dept Microbiol, A-6020 Innsbruck, Austria

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 09期

关键词：

D O I：

10.1093/nar/27.9.2057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhibitors of histone deacetylase (HD) bear great potential as new drugs due to their ability to modulate transcription and to induce apoptosis or differentiation in cancer cells. To study the activity of HD and the effect of potential inhibitors in vitro so far only radioactive assays have existed. For the search of new inhibitors and for the use in HD identification and purification we established a simple, non-radioactive assay that allows screening of large numbers of compounds. The assay is based on an aminocoumarin derivative of an Omega-acetylated lysine as enzyme substrate.

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页码：2057 / 2058

页数：2

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