Faster axial band dispersion in a monolithic silica column than in a particle-packed column

被引：50

作者：

Kobayashi, H

Tokuda, D

Ichimaru, J

Ikegami, T

Miyabe, K

Tanaka, N ^{[1
]}

机构：

[1] Kyoto Inst Technol, Dept Polymer Sci & Engn, Sakyo Ku, Kyoto 6068585, Japan

[2] Toyama Univ, Fac Engn, Dept Mat Syst Engn & Life Sci, Toyama 9308555, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2006年 / 1109卷 / 01期

关键词：

monolithic silica column; molecular diffusion; band dispersion;

D O I：

10.1016/j.chroma.2005.11.053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The contribution of molecular diffusion to peak broadening was studied in a reversed-phase HPLC system, consisting of a monolithic silica C Is column and methanol-water mobile phase. Study on the band broadening effect of holding a solute in a column or elution at very low linear velocity of mobile phase allowed facile determination of the contribution of the molecular diffusion term. Less obstruction against molecular diffusion, or the faster axial band dispersion in a monolithic silica column than in a particle-packed column, was found both in mobile phase and in stationary phase. (c) 2005 Elsevier B.V. All rights reserved.

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页码：2 / 9

页数：8

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