Strategies for accessing soil metagenome for desired applications

被引：101

作者：

Rajendhran, J. ^{[1
]}

Gunasekaran, P. ^{[1
]}

机构：

[1] Madurai Kamaraj Univ, Dept Genet, Ctr Excellence Genom Sci, Sch Biol Sci, Madurai 625021, Tamil Nadu, India

来源：

BIOTECHNOLOGY ADVANCES | 2008年 / 26卷 / 06期

关键词：

Metagenomics; Microbial diversity; Mobile genomics; Single cell genomics; Soil DNA; Sub-metagenomic library;

D O I：

10.1016/j.biotechadv.2008.08.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Most of the microorganisms in nature are inaccessible as they are uncultivable in the laboratory. Metagenomic approaches promise the accessibility of the genetic resources and their potential applications. Genetic resources from terrestrial environments can be accessed by exploring the soil metagenome. Soil metagenomic analyses are usually initiated by the isolation of environmental DNAs. Several methods have been described for the direct isolation of environmental DNAs from soil and sediments. Application of metagenomics largely depends on the construction of genomic DNA libraries and subsequent high-throughput sequencing or library screening. Thus, obtaining large quantities of pure cloneable DNA from the environment is a prerequisite. This review discusses the recent developments related to efficient extraction and purification of soil metagenome highlighting the considerations for various metagenomic applications. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：576 / 590

页数：15

共 140 条

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