Isolation of high molecular weight DNA from soil for cloning into BAC vectors

被引：50

作者：

Berry, AE

Chiocchini, C

Selby, T

Sosio, M

Wellington, EMH

机构：

[1] Univ Warwick, Dept Sci Biol, Coventry CV4 7AL, W Midlands, England

[2] Biosearch Italia, I-21040 Gerenzano, Italy

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 223卷 / 01期

关键词：

soil environment; DNA isolation; nycodenz gradient; bacterial artificial chromosome cloning; Escherichia coli-streptomycete artificial; chromosome;

D O I：

10.1016/S0378-1097(03)00248-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Isolation of high molecular weight DNA fragments from soil, in excess of 1 Mb, and of sufficient quality for cloning into an Escherichia coli-streptomycete artificial chromosome vector is described. The combination of indirect extraction of cells, using a nycodenz extraction technique, followed by lysis of biomass immobilised in agarose plugs, allowed fragments in excess of 1 Mb to be purified. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：15 / 20

页数：6

共 28 条

[1]

[Anonymous], 1995, METHODS APPL SOIL MI

[2]

Bakken L. R., 1995, Nucleic acids in the environment., P9

[3] Bacterial rhodopsin:: Evidence for a new type of phototrophy in the sea [J].

Béjà, O ;

Aravind, L ;

Koonin, EV ;

Suzuki, MT ;

Hadd, A ;

Nguyen, LP ;

Jovanovich, S ;

Gates, CM ;

Feldman, RA ;

Spudich, JL ;

Spudich, EN ;

DeLong, EF .

SCIENCE, 2000, 289 (5486) :1902-1906

[4] Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2) [J].

Bentley, SD ;

Chater, KF ;

Cerdeño-Tárraga, AM ;

Challis, GL ;

Thomson, NR ;

James, KD ;

Harris, DE ;

Quail, MA ;

Kieser, H ;

Harper, D ;

Bateman, A ;

Brown, S ;

Chandra, G ;

Chen, CW ;

Collins, M ;

Cronin, A ;

Fraser, A ;

Goble, A ;

Hidalgo, J ;

Hornsby, T ;

Howarth, S ;

Huang, CH ;

Kieser, T ;

Larke, L ;

Murphy, L ;

Oliver, K ;

O'Neil, S ;

Rabbinowitsch, E ;

Rajandream, MA ;

Rutherford, K ;

Rutter, S ;

Seeger, K ;

Saunders, D ;

Sharp, S ;

Squares, R ;

Squares, S ;

Taylor, K ;

Warren, T ;

Wietzorrek, A ;

Woodward, J ;

Barrell, BG ;

Parkhill, J ;

Hopwood, DA .

NATURE, 2002, 417 (6885) :141-147

[5] Search and discovery strategies for biotechnology: The paradigm shift [J].

Bull, AT ;

Ward, AC ;

Goodfellow, M .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2000, 64 (03) :573-+

[6] MATHEMATICAL-MODEL OF PLASMID TRANSFER BETWEEN STRAINS OF STREPTOMYCETES IN SOIL MICROCOSMS [J].

CLEWLOW, LJ ;

CRESSWELL, N ;

WELLINGTON, EMH .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (10) :3139-3145

[7] Quantification of bacterial subgroups in soil:: comparison of DNA extracted directly from soil or from cells previously released by density gradient centrifugation [J].

Courtois, S ;

Frostegård, Å ;

Göransson, P ;

Depret, G ;

Jeannin, P ;

Simonet, P .

ENVIRONMENTAL MICROBIOLOGY, 2001, 3 (07) :431-439

[8] BACTERIAL AND FUNGAL ACTIVITIES IN SOIL - SEPARATION OF BACTERIA AND FUNGI BY A RAPID FRACTIONATED CENTRIFUGATION TECHNIQUE [J].

FAEGRI, A ;

TORSVIK, VL ;

GOKSOYR, J .

SOIL BIOLOGY & BIOCHEMISTRY, 1977, 9 (02) :105-112

[9] Isolation of antibiotics turbomycin A and B from a metagenomic library of soil microbial DNA [J].

Gillespie, DE ;

Brady, SF ;

Bettermann, AD ;

Cianciotto, NP ;

Liles, MR ;

Rondon, MR ;

Clardy, J ;

Goodman, RM ;

Handelsman, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (09) :4301-4306

[10] Molecular biological access to the chemistry of unknown soil microbes: A new frontier for natural products [J].

Handelsman, J ;

Rondon, MR ;

Brady, SF ;

Clardy, J ;

Goodman, RM .

CHEMISTRY & BIOLOGY, 1998, 5 (10) :R245-R249

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