Development of wheat scab symptoms is delayed in transgenic wheat plants that constitutively express a rice thaumatin-like protein gene

被引:168
作者
Chen, WP
Chen, PD
Liu, DJ
Kynast, R
Friebe, B
Velazhahan, R
Muthukrishnan, S
Gill, BS [1 ]
机构
[1] Kansas State Univ, Wheat Genet Resource Ctr, Manhattan, KS 66506 USA
[2] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA
[3] Nanjing Agr Univ, Cytogenet Inst, Nanjing 210095, Jiangsu, Peoples R China
[4] Nanjing Agr Univ, Dept Agron, Nanjing 210095, Jiangsu, Peoples R China
[5] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA
关键词
Triticum aestivum; genetic transformation; thaumatin-like protein; wheat scab; fluorescent in situ hybridization;
D O I
10.1007/s001220051294
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The possibility of controlling wheat scab (caused by Fusarium graminearum Schw.) was explored by engineering wheat plants for constitutive expression of pathogenesis-related (PR) protein genes. A rice thaumatin-like protein (TLP) gene (tlp) and a rice chitinase gene (chill) were introduced into the spring wheat cultivar 'Bobwhite' by co-transformation of the plasmids pGL2ubi-tlp (ubiquitin/tlp//CaMV 35S/hpt) and pAHG11 (CaMV 35S/chi11//ubiquitin/bar). The transformation was by biolistic bombardment. Bialaphos was used as the selection reagent. The integration and expression of the tip, bar, chill and hpt genes were analyzed by Southern, Northern and Western blot analyses. The four transgenes co-segregated in the T-1 progeny of the transgenic plant and were localized at the telomeric region of the chromosome 6A long arm by sequential N-banding and fluorescent in situ hybridization (FISH) using pAHG11 or pGL2ubi-tlp as the probes. Only the transgenes tip and bar, under the control of the ubiquitin promoter-intron, were expressed. No expression of the chill and hpt genes, controlled by the CaMV 35S promoter, was detected in T-1 plants. After inoculation with conidia of F. graminearum, the symptoms of scab developed significantly slower in transgenic plants of the T-1, T-2 and T-3 generations expressing the tip gene than in non-transformed control plants. This is the first report of enhanced resistance to F. graminearum in transgenic wheat plants with constitutive expression of TLP.
引用
收藏
页码:755 / 760
页数:6
相关论文
共 40 条
[11]   Candidate gene analysis of quantitative disease resistance in wheat [J].
Faris, JD ;
Li, WL ;
Liu, DJ ;
Chen, PD ;
Gill, BS .
THEORETICAL AND APPLIED GENETICS, 1999, 98 (02) :219-225
[12]   Detection of single-copy genes and chromosome rearrangements in Petunia hybrida by fluorescence in situ hybridization [J].
Fransz, PF ;
Stam, M ;
Montijn, B ;
TenHoopen, R ;
Wiegant, J ;
Kooter, JM ;
Oud, O ;
Nanninga, N .
PLANT JOURNAL, 1996, 9 (05) :767-774
[13]   STANDARD KARYOTYPE AND NOMENCLATURE SYSTEM FOR DESCRIPTION OF CHROMOSOME BANDS AND STRUCTURAL-ABERRATIONS IN WHEAT (TRITICUM-AESTIVUM) [J].
GILL, BS ;
FRIEBE, B ;
ENDO, TR .
GENOME, 1991, 34 (05) :830-839
[14]   Field tolerance to fungal pathogens of Brassica napus constitutively expressing a chimeric chitinase gene [J].
Grison, R ;
GrezesBesset, B ;
Schneider, M ;
Lucante, N ;
Olsen, L ;
Leguay, JJ ;
Toppan, A .
NATURE BIOTECHNOLOGY, 1996, 14 (05) :643-646
[15]  
HESLOPHARRISON JS, 1991, TECHNIQUES, V3, P1
[16]   NUCLEOTIDE-SEQUENCE OF A RICE GENOMIC CLONE THAT ENCODES A CLASS-I ENDOCHITINASE [J].
HUANG, JK ;
WEN, L ;
SWEGLE, M ;
TRAN, HC ;
THIN, TH ;
NAYLOR, HM ;
MUTHUKRISHNAN, S ;
REECK, GR .
PLANT MOLECULAR BIOLOGY, 1991, 16 (03) :479-480
[17]   Visualization of a self-incompatibility gene in Brassica campestris L. by multicolor FISH [J].
Imano, M ;
Sakamoto, K ;
Suzuki, G ;
Watanabe, M ;
Takayama, S ;
Fukui, K ;
Hinata, K ;
Isogai, A .
THEORETICAL AND APPLIED GENETICS, 1998, 96 (6-7) :751-757
[18]   SEQUENTIAL CHROMOSOME-BANDING AND IN-SITU HYBRIDIZATION ANALYSIS [J].
JIANG, JM ;
GILL, BS .
GENOME, 1993, 36 (04) :792-795
[19]  
Jiang JM, 1996, MOL GEN GENET, V252, P497, DOI 10.1007/BF02172395
[20]   NONISOTOPIC IN-SITU HYBRIDIZATION AND PLANT GENOME MAPPING - THE FIRST 10 YEARS [J].
JIANG, JM ;
GILL, BS .
GENOME, 1994, 37 (05) :717-725