Investigation on Spectrum-Effect Correlation between Constituents Absorbed into Blood and Bioactivities of Baizhu Shaoyao San before and after Processing on Ulcerative Colitis Rats by UHPLC/Q-TOF-MS/MS Coupled with Gray Correlation Analysis

被引：52

作者：

Cai, Hao ^{[1
,2
]}

Xu, Yangyang ^{[1
,2
]}

Xie, Li ^{[1
,2
]}

Duan, Yu ^{[1
,2
]}

Zhou, Jia ^{[1
,2
]}

Liu, Jing ^{[1
,2
]}

Niu, Minjie ^{[1
,2
]}

Zhang, Yating ^{[1
,2
]}

Shen, Lin ^{[1
,2
]}

Pei, Ke ^{[3
]}

Cao, Gang ^{[4
]}

机构：

[1] Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210023, Jiangsu, Peoples R China

[2] Nanjing Univ Chinese Med, State Minist Educ Standardizat Chinese Med Proc, Engn Ctr, Nanjing 210023, Jiangsu, Peoples R China

[3] Shanxi Univ Tradit Chinese Med, Inst Pharmaceut & Food Engn, Taiyuan 030024, Shanxi, Peoples R China

[4] Zhejiang Chinese Med Univ, Sch Pharm, Hangzhou 310053, Zhejiang, Peoples R China

来源：

MOLECULES | 2019年 / 24卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Baizhu Shaoyao San; spectrum-effect correlation; UHPLC/Q-TOF-MS/MS; ulcerative colitis; gray correlation analysis; FLIGHT MASS-SPECTROMETRY; ATRACTYLODIS MACROCEPHALAE RHIZOMA; TRADITIONAL CHINESE MEDICINE; CHEMICAL DIFFERENTIATION; ATRACTYLENOLIDE I; DRUG DISCOVERY; CHROMATOGRAPHY; METABOLOMICS; PLASMA; SAPOSHNIKOVIAE;

D O I：

10.3390/molecules24050940

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Baizhu Shaoyao San (BSS) is a crucial traditional Chinese medicinal formula widely applied for the treatment of painful diarrhea, diarrhea-predominant irritable bowel syndrome, ulcerative colitis, and some other gastrointestinal diseases. Corresponding to the clinical medication, the three medicinal herbs (Atractylodis Macrocephalae Rhizoma, Paeoniae Radix Alba, and Citri Reticulatae Pericarpium) included in BSS should be processed using some specific methods of stir-frying. To find the underlying correlations between serum chemical profiles and curative effects of crude and processed BSS on ulcerative colitis rats, and further explore for the effective material basis of processing, an UHPLC/Q-TOF-MS/MS technique coupled with gray correlation analysis (GCA) was developed. A total of 134 compounds were identified in rat sera after oral administration of BSS, among which 24 compounds were prototypes and 110 compounds were metabolites. Meanwhile, an ulcerative colitis model was established in rats by enema with 2,4,6-trinitrobenzene sulfonic acid, and the pharmacodynamic indicators for drug efficacies were evaluated as well. According to the results, processed BSS showed better efficacy than crude BSS. The top 10 potential effective components with high degree of correlation were identified based on GCA results, which were thought to be the crucial compounds that contributed to the enhancement of therapeutic effects in BSS after processing.

引用

页数：26

共 49 条

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