Simultaneous reversed-phase high-performance liquid chromatographic separation of mono-, di-and trichloroanilines through a gradient elution optimised by experimental design

被引：21

作者：

Gennaro, MC ^{[1
]}

Marengo, E ^{[1
]}

Gianotti, V ^{[1
]}

Angioi, S ^{[1
]}

机构：

[1] Univ Piemonte Orientale, Amedeo Avogadro, Dipartimento Sci & Tecnol Avanzate, I-15100 Alessandria, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2002年 / 945卷 / 1-2期

关键词：

gradient elution; experimental design; chloranilines; anilines; amines;

D O I：

10.1016/S0021-9673(01)01487-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new RP-HPLC method for the simultaneous determination of the 13 mono-, di- and trichloroanilines has been developed. In order to obtain the analyte resolution within an acceptable analysis time, a gradient elution program has been optimised through the use of an experimental design and a grid search algorithm. The optimized conditions provided the resolution of all the analytes in less than 80 min. The primary validation of the analytical method gave limit of detection values ranging between 0.02 and 0.06 mg/l and very good linearity of the calibration curves. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：287 / 292

页数：6

共 21 条

[1]

BOX GEP, 1987, STAT EXPT

[2] REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF PESTICIDES .7. ANALYSIS OF VINCLOZOLIN, IPRODIONE, PROCYMIDONE, DICHLOROLINATE AND THEIR DEGRADATION PRODUCT 3,5-DICHLOROANILINE ON WHITE MUST AND WINE EXTRACTS [J].