Molecular cloning of the gyrA and gyrB genes of Bacteroides fragilis encoding DNA gyrase

被引：22

作者：

Onodera, Y ^{[1
]}

Sato, K ^{[1
]}

机构：

[1] Daiichi Pharmaceut Co Ltd, New Prod Res Labs 1, Edogawa Ku, Tokyo 1348630, Japan

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1999年 / 43卷 / 10期

关键词：

D O I：

10.1128/AAC.43.10.2423

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genes encoding the DNA gyrase A and B subunits of Bacteroides fragilis were cloned and sequenced. The gyrA and gyrB genes code for proteins of 835 and 653 amino acids, respectively. These proteins were expressed in Escherichia coli, and the combination of GyrA and GyrB exhibited ATP-dependent supercoiling activity. To analyze the role of DNA gyrase in quinolone resistance of B. fragilis, we isolated mutant strains by step,vise selection for resistance to increasing concentrations of levofloxacin. We analyzed the resistant mutants and showed that Ser-82 of GyrA, equivalent to resistance hot spot Ser-83 of GyrA in E. coli, was in each case replaced with Phe. These results suggest that DNA gyrase is an important target for quinolones in B. fragilis.

引用

页码：2423 / 2429

页数：7

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