RNA recognition and cleavage by a splicing endonuclease

被引:87
作者
Xue, S [1 ]
Calvin, K [1 ]
Li, H [1 ]
机构
[1] Florida State Univ, Inst Mol Biophys, Dept Chem & Biochem, Tallahassee, FL 32306 USA
关键词
D O I
10.1126/science.1126629
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The RNA splicing endonuclease cleaves two phosphodiester bonds within folded precursor RNAs during intron removal, producing the functional RNAs required for protein synthesis. Here we describe at a resolution of 2.85 angstroms the structure of a splicing endonuclease from Archaeglobus fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a cleaved splice site. The endonuclease dimer cooperatively recognized a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line RNA cleavage reaction. Cooperativity arises because an arginine pair from one catalytic domain sandwiches a nucleobase within the bulge cleaved by the other catalytic domain.
引用
收藏
页码:906 / 910
页数:5
相关论文
共 36 条
[1]   tRNA splicing [J].
Abelson, J ;
Trotta, CR ;
Li, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (21) :12685-12688
[2]   PARTICIPATION OF THE INTRON IN THE REACTION CATALYZED BY THE XENOPUS TRANSFER-RNA SPLICING ENDONUCLEASE [J].
BALDI, MI ;
MATTOCCIA, E ;
BUFARDECI, E ;
FABBRI, S ;
TOCCHINIVALENTINI, GP .
SCIENCE, 1992, 255 (5050) :1404-1408
[3]   Specificity of RNA-RNA helix recognition [J].
Battle, DJ ;
Doudna, JA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (18) :11676-11681
[4]   Probing the energetic and structural role of amino acid/nucleobase cation-π interactions in protein-ligand complexes [J].
Biot, C ;
Buisine, E ;
Kwasigroch, JM ;
Wintjens, R ;
Rooman, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (43) :40816-40822
[5]   IN-VITRO GENETIC-ANALYSIS OF THE STRUCTURAL FEATURES OF THE PRE-TRANSFER RNA REQUIRED FOR DETERMINATION OF THE 3' SPLICE-SITE IN THE INTRON EXCISION REACTION [J].
BUFARDECI, E ;
FABBRI, S ;
BALDI, MI ;
MATTOCCIA, E ;
TOCCHINIVALENTINI, GP .
EMBO JOURNAL, 1993, 12 (12) :4697-4704
[6]   Structural characterization of the catalytic subunit of a novel RNA splicing endonuclease [J].
Calvin, K ;
Hall, MD ;
Xu, FM ;
Xue, S ;
Li, H .
JOURNAL OF MOLECULAR BIOLOGY, 2005, 353 (05) :952-960
[7]   AN NAD DERIVATIVE PRODUCED DURING TRANSFER-RNA SPLICING - ADP-RIBOSE 1''-2'' CYCLIC PHOSPHATE [J].
CULVER, GM ;
MCCRAITH, SM ;
ZILLMANN, M ;
KIERZEK, R ;
MICHAUD, N ;
LAREAU, RD ;
TURNER, DH ;
PHIZICKY, EM .
SCIENCE, 1993, 261 (5118) :206-208
[8]   Solution structure of a substrate for the archaeal pre-tRNA splicing endonucleases: The bulge-helix-bulge motif [J].
Diener, JL ;
Moore, PB .
MOLECULAR CELL, 1998, 1 (06) :883-894
[9]   SUBSTRATE RECOGNITION AND IDENTIFICATION OF SPLICE SITES BY THE TRANSFER RNA-SPLICING ENDONUCLEASE AND LIGASE FROM SACCHAROMYCES-CEREVISIAE [J].
GREER, CL ;
SOLL, D ;
WILLIS, I .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (01) :76-84
[10]   MECHANISM OF ACTION OF A YEAST RNA LIGASE IN TRANSFER-RNA SPLICING [J].
GREER, CL ;
PEEBLES, CL ;
GEGENHEIMER, P ;
ABELSON, J .
CELL, 1983, 32 (02) :537-546