Determination of 1,4-benzodiazepines and their metabolites by capillary electrophoresis and high-performance liquid chromatography using ultraviolet and electrospray ionisation mass spectrometry

被引：68

作者：

McClean, S ^{[1
]}

O'Kane, E ^{[1
]}

Hillis, J ^{[1
]}

Smyth, WF ^{[1
]}

机构：

[1] Univ Ulster, ABCS Sch, Coleraine BT52 1SA, Londonderry, North Ireland

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 838卷 / 1-2期

关键词：

sample stacking; hair; benzodiazepines; diazepam; oxazepam; desmethyldiazepam; temazepam;

D O I：

10.1016/S0021-9673(99)00236-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A study is presented for the separation and determination of fifteen 1,4-benzodiazepine drugs and metabolites by capillary electrophoresis (CE) compared with high-performance liquid chromatography (HPLC). A comparison is made between the CE determination of the compounds by conventional UV detection and LC determination with electrospray ionisation (ESI) ion-trap mass spectrometry. CE is shown to provide superior separation to HPLC but the MS-MS capability of the ion-trap allows for the specific detection and determination of four of the compounds, diazepam, N'-desmethyldiazepam, oxazepam and temazepam in the hair of a patient under clinical treatment with diazepam and temazepam. Selected mixtures of drugs and metabolites are determined by CE and LC and the determination of diazepam and its metabolites by CE-UV-ESI-MS-MS is also presented. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：273 / 291

页数：19

共 49 条

[1] CHEMISTRY OF BENZODIAZEPINES [J].