Down-regulation of TGF-β1 production restores immunogenicity in prostate cancer cells

被引：37

作者：

Matthews, E. ^{[1
]}

Yang, T. ^{[1
]}

Janulis, L. ^{[1
]}

Goodwin, S. ^{[1
]}

Kundu, S. D. ^{[1
]}

Karpus, W. J. ^{[2
]}

Lee, C. ^{[1
]}

机构：

[1] Northwestern Univ, Sch Med, Dept Urol, Chicago, IL 60611 USA

[2] Northwestern Univ, Sch Med, Dept Pathol, Chicago, IL 60611 USA

来源：

BRITISH JOURNAL OF CANCER | 2000年 / 83卷 / 04期

关键词：

TGF-beta expression; rat prostate cancer; immunogenicity; tumour incidence; host-tumour interaction;

D O I：

10.1054/bjoc.2000.1257

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The objective of this study is to determine if a non-immunogenic Dunning's rat prostate cancer cell line, MATLyLu, can become immunogenic by reducing the endogenous production of TGF-beta 1. An expression construct containing a DNA sequence in an antisense orientation to TGF-beta 1 (TGF-beta 1 antisense) was stably transfected into MATLyLu cells. Following transfection, cellular content of TGF-beta 1 reduced from 70 to 10 pg per 2 x 10(4) cells and the rate of in vitro 3H-thymidine incorporation increased 3-5 fold. After subcutaneous injection of tumour cells into syngeneic male hosts (Copenhagen rats), the tumour incidence was 100% (15/15) for the wild type MATLyLu cells and cells transfected with the control construct, but only 43% (9/21, P <= 0.05) for cells transfected with TGF-beta 1 antisense. However, when cells were injected into immunodeficient hosts (athymic nude rats), the incidence of tumour development was 100% (10/10) for both the wild type MATLyLu cells and cells transfected with the control construct and 90% (9/10) for cells transfected with TGF-beta 1 antisense. These observations support the concept that MATLyLu cells are immunogenic, when the endogenous production of TGF-beta 1 is down-regulated. (C) 2000 Cancer Research Campaign

引用

页码：519 / 525

页数：7