A DSC investigation on the influence of gemini surfactant stereochemistry on the organization of lipoplexes and on their interaction with model membranes

被引：5

作者：

Aleandri, S. ^{[1
]}

Bonicelli, M. G. ^{[2
]}

Giansanti, L. ^{[1
,3
]}

Giuliani, C. ^{[1
]}

Ierino, M. ^{[2
]}

Mancini, G. ^{[1
,3
]}

Martino, A. ^{[1
]}

Scipioni, A. ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[2] Univ Roma La Sapienza, Dipartimento Ingn Chim Mat & Ambiente ICMA, I-00161 Rome, Italy

[3] Univ Roma La Sapienza, CNR, Ist Metodol Chim, I-00185 Rome, Italy

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2012年 / 165卷 / 08期

关键词：

Cationic lipoplex; Stereochemistry of gemini; DSC; Tm; Cell membrane model; Fusion; THERMOTROPIC PHASE-BEHAVIOR; ENHANCED GENE DELIVERY; TRANSFECTION EFFICIENCY; CHIRAL RECOGNITION; BIOMEMBRANE MODEL; DNA; CONDENSATION; AMPHIPHILES; COMPLEXES; ASSEMBLIES;

D O I：

10.1016/j.chemphyslip.2012.11.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Previous investigations showed that the extent of DNA condensation and the efficiency in the transfection of liposomes formulated with 1,2-dimyristoyl-sn-glycero-phosphocholine and cationic stereomeric gemini surfactants depend heavily on the stereochemistry of the gemini. The influence of the stereochemistry on the interaction of lipoplexes with zwitterionic and anionic cell membrane models was investigated by differential scanning calorimetry to rationalize their different biological behavior. Further, the thermotropic behavior of the corresponding liposomes and of the spontaneous self-assemblies of gemini surfactants in the presence and in the absence of DNA was evaluated to correlate the physicochemical properties of lipoplexes and the stereochemistry of the cationic component. The obtained results show that the stereochemistry of the gemini surfactant controls lipoplexes organization and their mode and kinetic of interaction with different cell membrane models. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：838 / 844

页数：7

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