Beneficial effect of autologous transplantation of bone marrow stromal cells and endothelial progenitor cells on cerebral ischemia in rabbits

被引：104

作者：

Chen, Zhen-Zhou ^{[1
]}

Jiang, Xiao-Dan ^{[1
]}

Zhang, Li-Li ^{[2
]}

Shang, Jiang-Hua ^{[1
]}

Du, Mou-Xuan ^{[1
]}

Xu, Gang ^{[1
]}

Xu, Ru-Xiang ^{[1
]}

机构：

[1] So Med Univ, Zhujiang Hosp, Dept Neurosurg, Guangzhou 510280, Guangdong, Peoples R China

[2] Guangzhou Univ Chinese Med, Dept ICU, Clifford Hosp, Guangzhou, Guangdong, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2008年 / 445卷 / 01期

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

Middle cerebral artery occlusion; Bone marrow stromal cells; Endothelial progenitor cells; Angiogenesis; Rabbits;

D O I：

10.1016/j.neulet.2008.08.039

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

We tested the therapeutic effect of autologous transplanted bone marrow stromal cells (BMSCs) and endothelial progenitor cells (EPCs) on cerebral ischemia in rabbits. Rabbit permanent middle cerebral artery occlusion (MCAO) models were intravenously injected with ex vivo expanded autologous BMSCs (n = 8), EPCs (n = 8), or phosphate-buffered saline (n = 6). 14 days after the transplantation, both infusion groups witnessed a functional improvement, a decrease in the number of apoptotic cells and an increase in the microvessel density in the ischemic boundary area, as compared to vehicle-treated control group. The EPCs treated group also exhibited a diminished infarct area in comparison with the control group. Moreover, immunohistochemistry revealed that few transplanted BMSCs expressed markets for astrocytes (GFAP(+)) and neurons (NeuN(+)), and most of EPCs were capable of binding to UEA-1 lectin and were incorporated into capillaries. Our data suggest that both BMSCs and EPCs, despite differences in their action mechanism, can be functional cytoreagents for treatment of cerebral ischemia in rabbit. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：36 / 41

页数：6

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