Transforming growth factor β controls the directional migration of hepatocyte cohorts by modulating their adhesion to fibronectin

被引：28

作者：

Biname, Fabien ^{[1
]}

Lassus, Patrice ^{[1
]}

Hibner, Urszula ^{[1
]}

机构：

[1] Univ Montpellier, CNRS, Inst Genet Mol, F-34293 Montpellier 5, France

来源：

MOLECULAR BIOLOGY OF THE CELL | 2008年 / 19卷 / 03期

关键词：

D O I：

10.1091/mbc.E07-09-0967

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Transforming growth factor beta(TGF-beta) has a strong impact on liver development and physiopathology, exercised through its pleiotropic effects on growth, differentiation, survival, and migration. When exposed to TGF-beta, the mhAT3F cells, immortalized, highly differentiated hepatocytes, maintained their epithelial morphology and underwent dramatic alterations of adhesion, leading to partial or complete detachment from a culture plate, followed by readhesion and spreading. These alterations of adhesive behavior were caused by sequential changes in expression of the alpha 5 beta 1 integrin and of its ligand, the fibronectin. The altered specificity of anchorage to the extracellular matrix gave rise to changes in cells' collective motility: cohorts adhering to fibronectin maintained a persistent, directional motility, with ezrin-rich pathfinder cells protruding from the tips of the cohorts. The absence of adhesion to fibronectin prevented the appearance of polarized pathfinders and lead to random, oscillatory motility. Our data suggest a novel role for TGF-beta in the control of collective migration of epithelial cohorts.

引用

页码：945 / 956

页数：12

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