An extended river buffalo (Bubalus bubalis, 2n=50) cytogenetic map:: assignment of 68 autosomal loci by FISH-mapping and R-banding and comparison with human chromosomes

被引：26

作者：

Di Meo, G. P. ^{[1
]}

Perucatti, A. ^{[1
]}

Floriot, S. ^{[2
]}

Hayes, H. ^{[2
]}

Schibler, L. ^{[2
]}

Incarnato, D. ^{[1
]}

Di Berardino, D. ^{[3
]}

Williams, J. ^{[4
]}

Cribiu, E. ^{[2
]}

Eggen, A. ^{[2
]}

Iannuzzi, L. ^{[1
]}

机构：

[1] ISPAAM, Natl Res Council, Lab Anim Cytogenet & Gene Mapping, I-80147 Naples, Italy

[2] INRA, UR339, F-78000 Jouy En Josas, France

[3] Univ Naples Federico II, Dept Soil Plant Environm & Anim Prod Sci, Portici, Italy

[4] Parco Tecnol Padano, Lodi, Italy

来源：

CHROMOSOME RESEARCH | 2008年 / 16卷 / 06期

关键词：

cytogenetic map; FISH; gene; human comparison; river buffalo;

D O I：

10.1007/s10577-008-1229-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report an extended river buffalo (Bubalus bubalis, 2n=50; BBU) cytogenetic map including 388 loci, of which 68 have been FISH-mapped on autosomes in the present study. Ovine and caprine BAC clones containing both type I loci (known genes) and type II loci (simple sequence repeats (SRs), microsatellite marker, sequence-tagged sites (STSs)), previously assigned to sheep chromosomes, have been localized on R-banded river buffalo chromosomes (BBU), which expands the cytogenetic map of this important domestic species and increases our knowledge of the physical organization of its genome. The loci mapped in the present study correspond to loci already localized on homoeologous cattle (and sheep) chromosomes and chromosome bands, further confirming the high degree of chromosome homoeologies among bovids. The comparison of the integrated cytogenetic maps of BBU2p/BBU10 and BBU5p/BBU16 with those of human chromosomes (HSA) 6 and 11, respectively, identified, at least, nine conserved chromosome segments in each case and complex rearrangements differentiating river buffalo (and cattle) and human chromosomes.

引用

页码：827 / 837

页数：11

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