In vitro analysis of ISEcp1B-mediated mobilization of naturally occurring β-lactamase gene blaCTX-M of Kluyvera ascorbata

被引:138
作者
Lartigue, MF [1 ]
Poirel, L [1 ]
Aubert, D [1 ]
Nordmann, P [1 ]
机构
[1] Univ Paris 11, Fac Med Paris Sud, Assistance Publ Hop Paris, Hop Bicetre,Serv Bacteriol Virol, F-94275 Le Kremlin Bicetre, France
关键词
D O I
10.1128/AAC.50.4.1282-1286.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
ISEcp1B has been reported to be associated with and to mobilize the emerging expanded-spectrum P-lactamase bla(CTX-M) genes in Enterobacteriaceae. Thus, the ability of this insertion sequence to mobilize the bla(CTX-M-2) gene was tested from its progenitor, Kluyvera ascorbata. Insertions of ISEcp1B upstream of the bla(CTX-M-2) gene in K. ascorbata reference strain CIP7953 were first selected with cefotaxime (0.5 and 2 mu g/ml). In those cases, ISEcp1B brought promoter sequences enhancing bla(CTX-M-2) expression in K. ascorbata. Then, ISEcp1B-mediated mobilization of the bla(CTX-M-2) gene from K. ascorbata to Escherichia coli J53 was attempted. The transposition frequency of ISEcp1B-bla(CTX-M-2) occurred at (6.4 +/- 0.5) x 10(-7) in E. coli. Cefotaxime, ceftazidime, and piperacillin enhanced transposition, whereas amoxicillin, cefuroxime, and nalidixic acid did not. Transposition was also enhanced when studied at 40 degrees C.
引用
收藏
页码:1282 / 1286
页数:5
相关论文
共 32 条
[1]   Ceftazidime-hydrolysing CTX-M-15 extended-spectrum β-lactamase (ESBL) in Poland [J].
Baraniak, A ;
Fiett, J ;
Hryniewicz, W ;
Nordmann, P ;
Gniadkowski, M .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2002, 50 (03) :393-396
[2]   A novel type of AmpC β-lactamase, ACC-1, produced by a Klebsiella pneumoniae strain causing nosocomial pneumonia [J].
Bauernfeind, A ;
Schneider, I ;
Jungwirth, R ;
Sahly, H ;
Ullmann, U .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (08) :1924-1931
[3]   SOS response promotes horizontal dissemination of antibiotic resistance genes [J].
Beaber, JW ;
Hochhut, B ;
Waldor, MK .
NATURE, 2004, 427 (6969) :72-74
[4]   Growing group of extended-spectrum β-lactamases:: The CTX-M enzymes [J].
Bonnet, R .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (01) :1-14
[5]   Identification and broad dissemination of the CTX-M-14 β-lactamase in different Escherichia coli strains in the northwest area of Spain [J].
Bou, G ;
Cartelle, M ;
Tomas, M ;
Canle, D ;
Molina, F ;
Moure, R ;
Eiros, JM ;
Guerrero, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) :4030-4036
[6]   ColE1-Like plasmid pIP843 of Klebsiella pneumoniae encoding extended-spectrum β-lactamase CTX-M-17 [J].
Cao, V ;
Lambert, T ;
Courvalin, P .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (05) :1212-1217
[7]   Three cefotaximases, CTX-M-9, CTX-M-13, and CTX-M-14, among Enterobacteriaceae in the People's Republic of China [J].
Chanawong, A ;
M'Zali, FH ;
Heritage, J ;
Xiong, JH ;
Hawkey, PM .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (03) :630-637
[8]  
Chandler M., 2002, Mobile DNA II, P305, DOI DOI 10.1128/9781555817954.CH15
[9]   Risk factors for the development of extended-spectrum beta-lactamase-producing bacteria in nonhospitalized patients [J].
Colodner, R ;
Rock, W ;
Chazan, B ;
Keller, N ;
Guy, N ;
Sakran, W ;
Raz, R .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2004, 23 (03) :163-167
[10]   GENETIC-ANALYSIS OF THE INTERACTION OF THE INSERTION-SEQUENCE IS903 TRANSPOSASE WITH ITS TERMINAL INVERTED REPEATS [J].
DERBYSHIRE, KM ;
HWANG, L ;
GRINDLEY, NDF .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (22) :8049-8053